Development of a multilayer microfluidic device integrated with a PDMS-cellulose composite film for sample pre-treatment and immunoassay
Development of a multilayer microfluidic device integrated with a PDMS-cellulose composite film for sample pre-treatment and immunoassay
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DOI：
10.1016/j.sna.2013.01.004
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发表时间：
2013-04
影响因子：
4.6
通讯作者：
Xing Chen;Lulu Zhang;Hui Li;Jianhan Sun;H. Cai;D. Cui
中科院分区：
文献类型：
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作者：
Xing Chen;Lulu Zhang;Hui Li;Jianhan Sun;H. Cai;D. Cui
本工作提出了一种集成的多层微流控装置，使原始样品的预处理和基于免疫测定的检测，其中聚二甲基硅氧烷（PDMS）-纤维素复合膜用于分离血浆从原始样品的基础上的交叉流的原则。采用浸涂法制备了聚二甲基硅氧烷-纤维素复合膜，通过扫描电镜观察了复合膜的形貌，并通过常压实验对复合膜的结合强度进行了定量分析。将该集成微流控装置用于检测大鼠全血样品中的人免疫球蛋白（IgG），定量检测范围为0.5-5μg/mL，相关系数为0.978。还通过集成的微流体装置测试了原始尿液样品，产生0.6-25μg/mL的检测范围，其中人IgG的相关系数为0.969。这种集成的微流体装置可以用作平台技术，使得能够现场检测原始临床样品。
This work presents an integrated multilayer microfluidic device enabling raw sample pre-treatment and immunoassay based detection where a polydimethylsiloxane (PDMS)-cellulose composite film was used to isolate plasma from raw samples based on the cross-flow principle. The PDMS-cellulose composite film was prepared by the dip-coating method and its morphologies were studied by the SEM technology and the bonding strength was quantified by atmospheric pressure experiments. The integrated microfluidic device was used to detect human immunoglobulin (IgG) in rat whole blood samples, with a quantified detection range of 0.5–5μg/mL and a correlation coefficient of 0.978. A raw urine sample was also tested by the integrated microfluidic device, producing a detection range of 0.6–25μg/mL with a correlation coefficient of 0.969 for human IgG. This integrated microfluidic device may function as a platform technology, enabling on-site detection of raw clinical samples.