Development of immune complex trapping: experimental study of lymphoid follicles and germinal centers newly induced by exogenous stimulants in mouse popliteal lymph nodes
Development of immune complex trapping: experimental study of lymphoid follicles and germinal centers newly induced by exogenous stimulants in mouse popliteal lymph nodes
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DOI：
10.14670/hh-14.11
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发表时间：
1999-01-01
影响因子：
2
通讯作者：
Hoshi, H
中科院分区：
文献类型：
--
作者：
Horie, K;Chen, D;Hoshi, H
在年轻的成年小鼠中研究了引流腘淋巴结的新诱导淋巴滤泡中免疫复合物捕获的发展，所述小鼠已被给予双侧注射血蓝蛋白（KLH）或植物血凝素（PHA），每种都被吸附到氧化铝上。处死前1天将HRP-抗HRP免疫复合物注射到足垫中。使用从每个腘淋巴结制备的三个系列的半连续低温恒温切片，对每个淋巴结中的淋巴滤泡的数量进行计数，并确定每个滤泡中体内注射和体外应用的免疫复合物的滤泡定位。到第5天，在每个先前存在的卵泡内已经形成了一个大的生发中心。大量的“新的”次级卵泡，每个包含一个小的PNA阳性生发中心，出现在预先存在的卵泡外，从第5天到第11天在KLH处理的节点，从第7天到第14天在PHA处理的节点。在它们出现后不久，新的次级卵泡没有显示出体外或体内捕获，但随后，许多新的卵泡开始显示体外捕获，起初很弱，但后来很强烈。新卵泡体内捕获的发生需要一些时间，当新卵泡在体外显示出强烈的捕获时，才首次发现。在第21或25天，许多新的卵泡显示出体外和体内捕获。得出的结论是，在用刺激剂处理的淋巴结中，含有生发中心的次级卵泡可以在没有卵泡捕获微环境的卵泡外区从头形成，但随后能够捕获免疫复合物的微环境在新卵泡形成的部位发育。
The development of immune complex trapping in newly-induced lymphoid follicles of draining popliteal lymph nodes was investigated in young adult mice, which had been given bilateral injection of hemocyanin (KLH) or phytohemagglutinin (PHA), each absorbed onto alumina. HRP-anti-HRP immune complex was injected into the footpad 1 day before sacrifice. Using three series of semi-serial cryostat sections prepared from each popliteal node, the number of lymphoid follicles in each node was counted, and follicular localization of the in vivo injected and in vitro applied immune complexes in each follicle was determined. By day 5, a large germinal center had developed within each preexisting follicle. A large number of 'new' secondary follicles, each containing a small PNA-positive germinal center, appeared outside pre-existing follicles, from day 5 through day 11 in KLH-treated nodes, and from day 7 through day 14 in PHA-treated nodes. Shortly after their appearance, new secondary follicles showed no in vitro or in vivo trapping, but subsequently, many of the new follicles began to display in vitro trapping, at first weakly but later intensely. Occurrence of in vivo trapping in new follicles took some time and was first recognized when new follicles showed intense in vitro trapping. At day 21 or 25, many of the new follicles showed both in vitro and in vivo trapping. It was concluded that in lymph nodes treated with a stimulant, secondary follicles containing germinal centers can be formed de novo in the extrafollicular zone where the follicular trapping microenvironment is absent, but subsequently the microenvironment capable of trapping immune complexes develops at the site of formation of new follicles.