Novel fluo-4 analogs for fluorescent calcium measurements
Novel fluo-4 analogs for fluorescent calcium measurements
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DOI：
10.1016/j.ceca.2004.05.002
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发表时间：
2004-12-01
期刊：
影响因子：
4
通讯作者：
Gee, KR
中科院分区：
文献类型：
--
作者：
Martin, VV;Beierlein, M;Gee, KR
我们报告新的荧光钙指示剂的基础上fluo-4。甲酰胺或亚甲基甲酰胺部分与fluo-4的BAPTA螯合剂部分的连接允许葡聚糖、蛋白质反应性部分和生物素的连接。特别是，制备了高亲和力的fluo-4葡聚糖缀合物，并显示其在脑切片中具有功能。所有新的探针进行了光谱表征，并表现出大的荧光增加后，钙结合。fluo-4的生物素化形式形成持久的链霉亲和素复合物，其仍然响应于增加的钙浓度，具有大的荧光增加。(C)2004爱思唯尔有限公司保留所有权利。
We report new fluorescent calcium indicators based on fluo-4. Attachment of a carboxamide or methylenecarboxamide moiety to the BAPTA chelator portion of fluo-4 allowed for the attachment of dextrans, protein-reactive moieties, and biotin. In particular, a high affinity fluo-4 dextran conjugate was prepared and shown to be functional in brain slices. All new probes were characterized spectroscopically and exhibited large fluorescence increases upon calcium-binding. The biotinylated version of fluo-4 formed a persistent streptavidin complex which still responded to increasing calcium concentrations with a large fluorescence increase. (C) 2004 Elsevier Ltd. All rights reserved.