Graphene oxide for solid-phase extraction of bioactive phenolic acids
Graphene oxide for solid-phase extraction of bioactive phenolic acids
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用于固相萃取生物活性酚酸的氧化石墨烯
DOI：
10.1007/s00216-017-0291-0
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发表时间：
2017-05-01
影响因子：
4.3
通讯作者：
Guo, Yong
中科院分区：
文献类型：
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作者：
Hou, Xiudan;Wang, Xusheng;Guo, Yong
建立了固相萃取(SPE)分析尿液中痕量酚酸(PAS、咖啡酸、阿魏酸、原儿茶酸、肉桂酸)的方法。在这项工作中，石墨烯氧化物(GO)涂层被接枝到纯二氧化硅上作为固相萃取材料进行研究。制备的GO表面具有层状皱折结构，粗糙有序，可以提供更开放的吸附位置。由于GO的亲水性和极性，GO对PAS的萃取效率高于还原GO，这与Gaussian09软件的理论计算结果一致。通过静态和动态吸附实验，研究了PAS在GO@Sil上的吸附机理，结果表明PAS在GO@Sil上为单层表面吸附。采用响应面方法优化了制备材料的萃取能力。在优化的条件下，原儿茶酸的线性范围为2-50μg，原儿茶酸的线性范围为1-50μg；咖啡酸、阿魏酸、肉桂酸的线性范围为1-50μg，L−1为1-50μg，检出限为0.2 5-1μg L−1。最后，将所建立的方法应用于两名健康志愿者的尿样分析。结果表明，所制备的材料是一种实用、经济的萃取和测定复杂基质中酚酸的介质。将氧化石墨烯作为固相萃取材料接枝到纯二氧化硅上，用于尿液中酚酸的萃取，并对其萃取机理进行了研究。
A solid-phase extraction (SPE) method for the efficient analysis of trace phenolic acids (PAs, caffeic acid, ferulic acid, protocatechuic acid, cinnamic acid) in urine was established. In this work, a graphene oxide (GO) coating was grafted onto pure silica to be investigated as SPE material. The prepared GO surface had a layered and wrinkled structure that was rough and well organized, which could provide more open adsorption sites. Owing to its hydrophilicity and polarity, GO showed higher extraction efficiency toward PAs than reduced GO did, in agreement with the theoretical calculation results performed by Gaussian 09 software. The adsorption mechanism of PAs on GO@Sil was also investigated through static state and kinetic state adsorption experiments, which showed a monolayer surface adsorption. Extraction capacity of the as-prepared material was optimized using the response surface methodology. Under the optimized conditions, the as-established method provided wide linearity range (2–50 μg L−1for protocatechuic acid and 1–50 μg L−1for caffeic acid, ferulic acid, and cinnamic acid) and low limits of detection (0.25–1 μg L−1). Finally, the established method was applied for the analysis of urine from two healthy volunteers. The results indicate that the prepared material is a practical, cost-effective medium for the extraction and determination of phenolic acids in complex matrices.Graphical AbstractA graphene oxide coating was grafted onto pure silica as the SPE material for the extraction of phenolic acids in urines and the extraction mechanism was also mainly investigated.