Circadian dynamics of vasopressin in mouse selection lines: translation and release in the SCN.
Circadian dynamics of vasopressin in mouse selection lines: translation and release in the SCN.
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小鼠选择系中加压素的昼夜动态：SCN 中的翻译和释放。
DOI：
10.1016/j.brainres.2005.07.068
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Bult-Ito,A
中科院分区：
文献类型：
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作者：
VanderVeen,DR;Castillo,MR;VanderZee,EA;Jansen,K;Gerkema,MP;Bult-Ito,A
arg8 -抗利尿激素（AVP）是一种生物钟控制的基因产物，在小鼠下丘脑视交叉上核（SCN）中以昼夜节律方式释放。先前报道的两种小鼠系的差异，最初被选择为热调节筑巢行为（建造小巢（s -小鼠）或大巢（b -小鼠）），具有不同的昼夜节律组织行为和SCN-AVP免疫反应神经元的数量，被进一步研究。我们证实并扩展了这一发现，即s型小鼠表现出持续高水平的SCN-AVP含量，没有明显的昼夜节律性，而b型小鼠的AVP阳性细胞数量较低，且随时间变化。我们发现AVP mRNA在午夜和中午的表达水平相似，通过原位杂交确定。当秋水仙碱阻断AVP的转运和释放时，两种细胞系的SCN-AVP免疫反应性相似。这表明SCN-AVP含量的差异取决于转运或释放。器官型SCN培养的b -小鼠比s -小鼠的AVP释放量更多。这些结果表明，在机制水平上，小鼠系在SCN中转运和/或释放AVP方面存在差异，而不是AVP基因转录或AVP免疫反应神经元数量的差异调节。
Arg8-vasopressin (AVP), a circadian clock-controlled gene product, is released from the hypothalamic suprachiasmatic nuclei (SCN) in mice in a circadian fashion. Previously reported differences in two mouse lines, initially selected for thermoregulatory nest-building behavior (building small nests (S-mice) or big nests (B-mice)) with different circadian organization of behavior and in number of SCN-AVP immunoreactive neurons, were further investigated. We confirmed and expanded the finding that S-mice exhibited constant high levels of SCN-AVP content with no apparent circadian rhythmicity, whereas B-mice had lower numbers of AVP positive cells which varied with time of day. We found that AVP mRNA expression levels at midnight and midday were similar in both lines, as established by in situ hybridization. When AVP transport and release were blocked by colchicine, SCN-AVP immunoreactivity was similar in both lines. This suggests that differences in SCN-AVP content depend on transport or release. Organotypic SCN cultures of B-mice showed more AVP release per neuron than cultures of S-mice. These results reveal that on a mechanistic level the mouse lines differed in transport and/or release of AVP in the SCN, rather than differential regulation of AVP gene transcription or number of AVP immunoreactive neurons.