Control of the action of phospholipases A by "vertical compression" of the substrate monolayer.
Control of the action of phospholipases A by "vertical compression" of the substrate monolayer.
复制标题
通过底物单层的“垂直压缩”控制磷脂酶 A 的作用。
DOI：
10.1021/bi00392a035
复制
发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
P. Kinnunen
中科院分区：
文献类型：
--
作者：
T. Thuren;J. Virtanen;P. Kinnunen
在空气-水界面处的外消旋-1，2-双十二烷酰基-sn-甘油基-3-磷酸甘油的单层通过用烷基化玻璃板代替空气同时保持15 mN m-1的恒定表面压力来“垂直压缩”。在该表面压力下，脂质膜被烷基化表面覆盖导致驻留在水和烷基化玻璃之间的界面处的那些磷脂分子的平均分子面积平均增加16 A2/分子。随后，磷脂酶A1和A2对单层的活动进行了测量，在存在和不存在的支持。虽然磷脂酶A1的活性增加4倍的支持，磷脂酶A2的活性降低到15%的烷基化表面的情况下测量的活性。这些发现表明，这种“垂直压缩”的单层可能会引起磷脂分子的构象变化，这反过来又会导致上述的磷脂酶A1和A2的活性的相互变化。一个分子模型占这些研究结果。
Monolayers of rac-1,2-didodecanoyl-sn-glycero-3-phosphoglycerol at an air-water interface were "vertically compressed" by substituting an alkylated glass plate for air while maintaining a constant surface pressure of 15 mN m-1. At this surface pressure the overlaying of the lipid film by the alkylated surface resulted in an average increase of 16 A2/molecule in the mean molecular area of those phospholipid molecules residing at the interface between water and the alkylated glass. Subsequently, the activities of phospholipases A1 and A2 toward the monolayers were measured both in the presence and in the absence of the support. While phospholipase A1 activity was increased 4-fold by the support, the activity of phospholipase A2 was reduced to 15% of the activity measured in the absence of the alkylated surface. These findings indicate that such a "vertical compression" of the monolayer is likely to induce a conformational change in the phospholipid molecules, which in turn would cause the above reciprocal changes in the activities of phospholipases A1 and A2. A molecular model accounting to these findings is presented.