Positive selection of mouse NK1+ T cells by CD1-expressing cortical thymocytes.
Positive selection of mouse NK1+ T cells by CD1-expressing cortical thymocytes.
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DOI：
10.1084/jem.182.6.2091
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发表时间：
1995-12-01
期刊：
影响因子：
--
通讯作者：
Bendelac A
中科院分区：
文献类型：
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作者：
Bendelac A
小鼠NK 1 + T细胞构成α/β TCR+ T细胞的亚群，其专门快速产生细胞因子，特别是IL-4，并可促进Th 2型CD 4 T细胞的分化。他们的TCR，就像人类T细胞的同源亚群一样，使用不变的TCR α链，最近被证明对β 2-微球蛋白相关的MHC I类CD 1分子具有特异性，这些分子在MHC外编码。与主流胸腺细胞（其识别胸腺上皮细胞上的阳性选择MHC配体）相反，NK 1 + T细胞的阳性选择需要其CD 1配体在骨髓源性细胞上表达。为了研究所涉及的骨髓衍生细胞的性质，用来自正常、SCID和MHC缺陷小鼠的组织构建嵌合小鼠，使得CD 1可以由胸腺中骨髓衍生细胞的不同亚群选择性地表达。还使用抗CD 1 mAb和CD 1特异性T细胞杂交瘤直接评估CD 1表达。结果表明，未成熟的（CD 4 +8+双阳性）皮质胸腺细胞是来源的CD 1呈递NK 1 + T细胞的阳性选择。
Mouse NK1+ T cells constitute a subset of alpha/beta TCR+ T cells that specialize in the rapid production of cytokines, in particular IL-4, and may promote the differentiation of Th2-type CD4 T cells. Their TCRs, like those of a homologous subset of human T cells, use an invariant TCR alpha chain and were recently shown to be specific for the beta 2-microglobulin-associated, MHC class I-like CD1 molecules, which are encoded outside the MHC. In contrast to mainstream thymocytes, which recognize their positively selecting MHC ligand on thymic epithelial cells, positive selection of NK1+ T cells requires their CD1 ligand to be expressed on bone marrow-derived cells. To investigate the nature of the bone marrow-derived cell involved, chimeric mice were constructed with tissues from normal, SCID, and MHC- deficient mice, so that CD1 could be selectively expressed by different subsets of bone marrow-derived cells in the thymus. CD1 expression was also directly assessed using an anti-CD1 mAb, and a CD1-specific T cell hybridoma. The results suggest that immature (CD4+8+ double-positive) cortical thymocytes are the source of CD1 presentation for positive selection of NK1+ T cells.