Ovarian aromatase loss-of-function mutant medaka undergo ovary degeneration and partial female-to-male sex reversal after puberty
Ovarian aromatase loss-of-function mutant medaka undergo ovary degeneration and partial female-to-male sex reversal after puberty
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DOI：
10.1016/j.mce.2017.07.013
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发表时间：
2018-01
影响因子：
4.1
通讯作者：
M. Nakamoto;Y. Shibata;K. Ohno;T. Usami;Y. Kamei;Yoshihito Taniguchi;T. Todo;T. Sakamoto;G. Young;P. Swanson;K. Naruse;Y. Nagahama
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文献类型：
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作者：
M. Nakamoto;Y. Shibata;K. Ohno;T. Usami;Y. Kamei;Yoshihito Taniguchi;T. Todo;T. Sakamoto;G. Young;P. Swanson;K. Naruse;Y. Nagahama
虽然雌激素被普遍认为在非哺乳动物的卵巢分化中起着关键作用，但雌激素在卵巢分化过程中的具体功能尚不清楚。我们从稻科植物（Oryzias latipes）的n -乙基-n -亚硝基源基因驱动诱变文库中分离出两个卵巢芳香化酶（cyp19a1）基因过早停止的突变体。在XX突变体中，性腺首先分化为含有许多卵泡的正常卵巢，这些卵泡不能积累蛋黄。随后，卵巢组织发生广泛变性，随后出现卵巢背侧的睾丸组织。在新形成的睾丸组织中，在种系干细胞周围的sox9a2阳性体细胞中检测到gsdf的强表达，这表明gsdf在雌激素缺乏的雌性向雄性转变的睾丸分化过程中发挥了重要作用。我们得出结论，受精后合成的内源性雌激素对卵巢早期分化不是必需的，但对维持成年卵巢至关重要。
Although estrogens have been generally considered to play a critical role in ovarian differentiation in non-mammalian vertebrates, the specific functions of estrogens during ovarian differentiation remain unclear. We isolated two mutants with premature stops in the ovarian aromatase (cyp19a1) gene from an N-ethyl-N-nitrosourea-based gene-driven mutagenesis library of the medaka,Oryzias latipes. In XX mutants, gonads first differentiated into normal ovaries containing many ovarian follicles that failed to accumulate yolk. Subsequently, ovarian tissues underwent extensive degeneration, followed by the appearance of testicular tissues on the dorsal side of ovaries. In the newly formed testicular tissue, strong expression ofgsdfwas detected insox9a2-positive somatic cells surrounding germline stem cells suggesting thatgsdfplays an important role in testicular differentiation during estrogen-depleted female-to-male sex reversal. We conclude that endogenous estrogens synthesized after fertilization are not essential for early ovarian differentiation but are critical for the maintenance of adult ovaries.