Gene Expression of Haloferax volcanii on Intermediate and Abundant Sources of Fixed Nitrogen
Gene Expression of Haloferax volcanii on Intermediate and Abundant Sources of Fixed Nitrogen
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DOI：
10.3390/ijms20194784
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发表时间：
2019-10-01
影响因子：
5.6
通讯作者：
Maupin-Furlow, Julie A.
中科院分区：
文献类型：
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作者：
Hwang, Sungmin;Chavarria, Nikita E.;Maupin-Furlow, Julie A.
火山卤代古菌是一种发育良好的古菌模型，可用于基因组学、转录组学和蛋白质组学分析，它可以在固定氮丰富和中等水平的培养基上生长。在这里，我们报告了一项全球基因表达分析，与l-丙氨酸相比，生长在铵上的H. volcanii作为丰富的固定氮来源，后者是一种可以从自然栖息地的死细胞中获得氮的中间来源。通过比较两种生长条件，发现30个基因存在差异表达，其中16个基因与氨基酸代谢和转运相关。基因表达谱有助于定位铵和l-丙氨酸在转运体和代谢途径方面的使用。此外，在假定的启动子区域中发现了保守的DNA基序，并且发现转录因子与差异表达的基因是一致的，这使我们提出了转录共调控操纵子的调控。这项研究为H. volcanii如何响应和利用中间和丰富的固定氮源进行生长提供了见解，并对相关嗜盐古菌的保守功能产生了影响。
Haloferax volcanii, a well-developed model archaeon for genomic, transcriptomic, and proteomic analyses, can grow on a defined medium of abundant and intermediate levels of fixed nitrogen. Here we report a global profiling of gene expression of H. volcanii grown on ammonium as an abundant source of fixed nitrogen compared to l-alanine, the latter of which exemplifies an intermediate source of nitrogen that can be obtained from dead cells in natural habitats. By comparing the two growth conditions, 30 genes were found to be differentially expressed, including 16 genes associated with amino acid metabolism and transport. The gene expression profiles contributed to mapping ammonium and l-alanine usage with respect to transporters and metabolic pathways. In addition, conserved DNA motifs were identified in the putative promoter regions and transcription factors were found to be in synteny with the differentially expressed genes, leading us to propose regulons of transcriptionally co-regulated operons. This study provides insight to how H. volcanii responds to and utilizes intermediate vs. abundant sources of fixed nitrogen for growth, with implications for conserved functions in related halophilic archaea.