SNAREs and NSF in targeted membrane fusion
SNAREs and NSF in targeted membrane fusion
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DOI：
10.1016/s0955-0674(97)80026-9
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发表时间：
1997-08-01
影响因子：
7.5
通讯作者：
Scheller, RH
中科院分区：
文献类型：
--
作者：
Hay, JC;Scheller, RH
目前囊泡运输中的一个主要问题是，在SNARE(SNAP受体)复合体形成之前，或者在接近膜融合的步骤对接之后，是否需要NSF(N-乙基马来酰亚胺敏感因子)和α-SNAP(α-可溶性NSF连接蛋白)。最近对酵母空泡融合的研究表明，在SNAR对接复合体组装之前，对ATP、NSF和a-SNAP的要求可以完全满足；然而，这些因素的预对接作用的普遍性仍有待确定。液泡融合系统也被用来直接证明两种融合膜上对SNARE蛋白的需求，验证了当前融合模型的一个中心假设。
A major current issue in vesicle trafficking is whether NSF (N-ethylmaleimide-sensitive-factor) and alpha-SNAP (alpha-soluble NSF attachment protein) are required prior to SNARE (SNAP receptor) complex formation to allow vesicle docking, or after docking at a step close to membrane fusion. Recent studies of yeast vacuolar fusion indicated that the requirement for ATP, NSF and a-SNAP could be completely satisfied prior to SNARE docking complex assembly; however, the universality of a predocking role for these factors remains to be established. The vacuolar fusion system has also been used to directly demonstrate a requirement for SNARE proteins on both fusing membranes, verifying a central postulate of current fusion models.