DILATED CISTERNAE IN CAPPARALES - ATTEMPT TOWARDS THE CHARACTERIZATION OF A SPECIFIC ENDOPLASMIC-RETICULUM
DILATED CISTERNAE IN CAPPARALES - ATTEMPT TOWARDS THE CHARACTERIZATION OF A SPECIFIC ENDOPLASMIC-RETICULUM
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DOI：
10.1007/bf01276292
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发表时间：
1978-01-01
期刊：
影响因子：
2.9
通讯作者：
ESCHLBECK, G
中科院分区：
文献类型：
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作者：
BEHNKE, HD;ESCHLBECK, G
内质网扩张池（DC）是十字花科（14种）茎、下胚轴、根维管薄壁组织和根冠细胞的典型组成部分。筛选）和山柑科（7种）。一般来说，DC是非常长的，含有蛋白质的椭圆形细胞器，并由布满核糖体的单位膜结合。在所测试的植物的地上部分的蛋白质是平行于DC轴排列的管状结构，在根中有丝状蛋白。在一些菊科（例如，Lunaria，Ptilotrichum，Schivereckia）DC形状不规则，含有颗粒状蛋白质物质。这些形式可能导致在Tovaria的伴随细胞中发现的含蛋白质的空泡。由于DC确实存在于含有硫代葡萄糖苷的分类群中，因此对β-DC进行EM细胞化学测试是可行的。硫代葡糖苷酶应用于一些物种。结果并没有证明DC内的酶的特定位置。该方法的可靠性受到质疑，DC是β-β位点的概率也受到质疑。讨论硫葡萄糖苷酶是因为已知，例如，含芥子油苷芦苇科不含这种细胞器。红木属（Erythroxylaceae）和蓝花楹属（Jacaranda）在进行细胞化学测试时给出了相同的沉淀。
Dilated cisternae (DC) of endoplasmic reticulum were a typical component of stem-, hypocotyledon- and root vascular parenchyma and root cap cells in Brassicaceae (14 spp. screened) and Capparaceae (7 spp.). In general, DC are very long, utricular organelles containing proteins and bound by a unit membrane which is studded with ribosomes. In aerial parts of the plants tested the proteins are tubular structures arranged parallel to the DC axis, in roots there are filamentous proteins. In some Brassicaceae (e.g., Lunaria, Ptilotrichum, Schivereckia) DC are irregularly shaped and contain granular protein material. These forms may lead to protein-containing vacuoles as found in companion cells of Tovaria. Since DC do occur in taxa which contain glucosinolates, an EM-cytochemical test for .beta.-thioglucosidases was applied to some of the species. The results did not prove a specific location of the enzyme inside DC. The reliability of the method is questioned and the probability that DC are a site of .beta.-thioglucosidases is discussed since it is known that, e.g., the glucosinolate-containing Resedaceae do not contain this organelle. Erythroxylum (Erythroxylaceae) and Jacaranda (Bignoniaceae) gave the same precipitations when subjected to the cytochemical test.