Application of water mimics on preparation of eicosapentaenoic and docosahexaenoic acids containing glycerolipids
Application of water mimics on preparation of eicosapentaenoic and docosahexaenoic acids containing glycerolipids
复制标题
水模拟物在制备含甘油脂的二十碳五烯酸和二十二碳六烯酸中的应用
DOI：
10.1007/bf02636082
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
M. Hatano
中科院分区：
文献类型：
--
作者：
M. Hosokawa;Koretarō Takahashi;Noriko Miyazaki;K. Okamura;M. Hatano
为了提高高不饱和脂肪酸的掺入率和甘油脂产物的回收率，用高介电常数的有机溶剂(模拟水)代替部分脂肪酶必需水来研究它们对酸解和酯交换反应的影响。在鱼油甘油三酯与二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)的酸解/酯交换反应中，Lipozyme IM-60以乙二醇为模拟水剂，促进了EPA的掺入，抑制了合成甘油脂的水解。另一方面，水和丙二醇组分促进了大豆磷脂酰胆碱和EPA之间的酯交换反应。在含有适量水和有机溶剂(模拟水)的非水介质中，Lipozyme IM-60可促进EPA的酯交换反应生成大豆磷脂酰胆碱。同时，回收的甘油脂产品对新合成的含有EPA和DHA的甘油脂的水解率降低。
To obtain enhanced incorporation of highly unsaturated fatty acids and recovery of glycerolipid products, organic solvents with high dielectric constants (water mimics) were substituted for part of the essential water for lipase activation to study their effect on acidolysis and transesterification. In acidolysis/transesterification of fish oil triglycerides and eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), Lipozyme IM-60 with ethylene glycol as a water mimic enhanced the incorporation of EPA and suppressed the hydrolysis of synthesized glycerolipid. On the other hand, transesterification between soy phosphatidylcholine and EPA was enhanced by a water and propylene glycol combination. In a nonaqueous medium that contained appropriate amounts of water and organic solvents (water mimics), Lipozyme IM-60 increased transesterification of EPA into soy phosphatidylcholine. Simultaneously, the recovered glycerolipid products showed decreased hydrolysis of newly synthesized EPA- and DHA-containing glycerolipids.