Characterization of bacteriophage vB_KleM_KB2 possessing high control ability to pathogenic Klebsiella pneumoniae.
Characterization of bacteriophage vB_KleM_KB2 possessing high control ability to pathogenic Klebsiella pneumoniae.
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DOI：
10.1038/s41598-023-37065-5
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发表时间：
2023-06-17
影响因子：
4.6
通讯作者：
Yuan, Yihui
中科院分区：
文献类型：
--
作者：
Peng, Qin;Ma, Zimeng;Han, Qing;Xiang, Fangfang;Wang, Lushuang;Zhang, Yibin;Zhao, Yuting;Li, Jianing;Xian, Yaxin;Yuan, Yihui
肺炎克雷伯氏菌是多种人类疾病的广泛病原体。多药耐药K.肺炎使得这些疾病的治疗成为一个重大挑战。噬菌体的应用是应对多重耐药病原菌的潜在途径。本研究分离了一种新的噬菌体vB_KleM_KB2，该噬菌体感染多重耐药的临床分离株。肺炎。该噬菌体的潜伏期较短，仅为10 min，60 min内即可有效裂解细菌。值得注意的是，噬菌体可以在107 CFU/mL的初始浓度下完全抑制宿主细菌的生长，感染复数为0.001，这证明了其高裂解活性。此外，噬菌体显示出高的环境耐受性，这可以促进噬菌体的实际应用。对噬菌体基因组的分析表明，该噬菌体具有新的基因组序列，可以代表一个新的噬菌体属。噬菌体vB_KleM_KB2具有裂解活性高、潜伏期短、稳定性好、遗传背景新颖等特点，丰富了噬菌体库，为多重耐药病原菌的防治提供了新的途径。肺炎。
Klebsiella pneumoniae is a widespread pathogen of several human diseases. The emergence of multidrug-resistant K. pneumoniae makes the treatment of these diseases a significant challenge. The application of bacteriophages is a potential approach for dealing with the emergence of multidrug-resistant pathogenic bacteria. This study isolates a novel bacteriophage vB_KleM_KB2 that infects the multidrug-resistant clinical isolates of K. pneumoniae. The bacteriophage exhibits a short latent period of 10 min, and can effectively lyse the bacterium within 60 min. Notably, the bacteriophage can completely inhibit the growth of the host bacterium at the initial concentration of 107 CFU/mL with a low multiplicity of infection of 0.001, which proves its high lytic activity. Furthermore, the bacteriophage shows high environmental tolerances, which can facilitate the practical application of the bacteriophage. Analysis of the bacteriophage genome shows that the bacteriophage possesses a novel genome sequence and can represent a new bacteriophage genus. Considering the high lytic activity, short latent period, high stability, and novel genetic background, bacteriophage vB_KleM_KB2 enriches the bacteriophage library and provides a new alternative for controlling the diseases caused by multidrug-resistant pathogenic K. pneumoniae.
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影响因子：
14.2
作者：
Magiorakos, A. -P.;Srinivasan, A.;Monnet, D. L.
通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
LEVIN, BR
影响因子：
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作者：
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通讯作者：
Tripathi, B. N.