Sampling methodologies and dosage assessment techniques for submicrometre and ultrafine virus aerosol particles
Sampling methodologies and dosage assessment techniques for submicrometre and ultrafine virus aerosol particles
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DOI：
10.1111/j.1365-2672.2005.02720.x
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发表时间：
2005-01-01
影响因子：
4
通讯作者：
Biswas, P
中科院分区：
文献类型：
--
作者：
Hogan, CJ;Kettleson, EM;Biswas, P
目的：研究亚微米和超细病毒粒子的气雾化和收集，目的是建立稳健和准确的方法来研究空气中的病毒粒子。方法和结果：评估了三种用于采样空气中的生物粒子的采样设备-全玻璃Impinger 30、SKC生物采样器(R)和弗里特鼓泡器-对亚微米和超细病毒粒子的收集效率。通过雾化单链RNA和双链DNA噬菌体的悬浮液来产生测试病毒气溶胶颗粒。粒度分布结果表明，典型的病毒悬浮液中存在的病毒比例极低，病毒的存在对雾化悬浮液的粒度分布几乎没有影响。结果发现，所有被测试的取样器都不足以收集亚微米和超细的病毒颗粒，在30-100 nm的尺寸范围内，所有取样器的收集效率都低于10%。空斑分析和颗粒计数测量表明，所有被测试的取样器都具有随时间变化的病毒颗粒采集效率。结论：差示迁移率分析与传统空斑分析技术相结合，可用于全面表征空气传播病毒。本研究的意义和影响：所提供的数据和方法为未来亚微米和超细空气传播病毒粒子的研究提供了基本的基础。
Aims: The aerosolization and collection of submicrometre and ultrafine virus particles were studied with the objective of developing robust and accurate methodologies to study airborne viruses.Methods and Results: The collection efficiencies of three sampling devices used to sample airborne biological particles - the All Glass Impinger 30, the SKC BioSampler (R) and a frit bubbler - were evaluated for submicrometre and ultrafine virus particles. Test virus aerosol particles were produced by atomizing suspensions of single-stranded RNA and double-stranded DNA bacteriophages. Size distribution results show that the fraction of viruses present in typical aqueous virus suspensions is extremely low such that the presence of viruses has little effect on the particle size distribution of atomized suspensions. It has been found that none of the tested samplers are adequate in collecting submicrometre and ultrafine virus particles, with collection efficiencies for all samplers below 10% in the 30-100 nm size range. Plaque assays and particle counting measurements showed that all tested samplers have time-varying virus particle collection efficiencies. A method to determine the size distribution function of viable virus containing particles utilizing differential mobility selection was also developed.Conclusions: A combination of differential mobility analysis and traditional plaque assay techniques can be used to fully characterize airborne viruses.Significance and Impact of the Study: The data and methods presented here provide a fundamental basis for future studies of submicrometre and ultrafine airborne virus particles.