Study of differential collagen synthesis during development of the chick embryo by immunofluorescence: II. Localization of type I and type II collagen during long bone development
Study of differential collagen synthesis during development of the chick embryo by immunofluorescence: II. Localization of type I and type II collagen during long bone development
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鸡胚发育过程中胶原蛋白合成差异的免疫荧光研究：II．
DOI：
10.1016/0012-1606(76)90220-7
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发表时间：
1976
影响因子：
2.7
通讯作者：
H. Mark
中科院分区：
文献类型：
--
作者：
K. Mark;H. Mark
用免疫荧光法研究了鸡胚软骨和骨发育过程中I型和II型胶原的转变。在所有软骨结构中发现II型胶原，其显示异染性染色。I型胶原蛋白出现在软骨膜的胫骨在第28阶段，也被发现在类骨质，骨膜和enchondrification骨脱钙后，骨膜，肌腱，韧带，和capsule.Using免疫组化方法，它是可能的，以确定特定的胶原蛋白类型的地区进行快速增殖和胶原蛋白的过渡，如骨干和骺软骨膜，或enchondrification成骨。在软骨内骨化过程中，I型胶原沉积在软骨的侵蚀表面上。它部分扩散到软骨基质中，形成具有II型胶原的“混合”胶原基质，这是随后骨化的部位。在骨干软骨的贴壁生长和骺软骨膜分化成关节软骨的过程中，软骨膜细胞在分化成成软骨细胞时从I型胶原合成转变为II型胶原合成。在过渡区，成软骨细胞嵌入由I型和II型胶原混合物组成的“混合”基质中。
The transition of type I and type II collagens during cartilage and bone development in the chick embryo was studied by immunofluorescence using antibodies against type I or type II collagens. Type II collagen was found in all cartilaginous structures which showed metachromatic staining. Type I collagen appeared in the perichondrium of the tibia at stage 28 and was also found in osteoid, periosteal and enchondral bone after decalcification, periosteum, and tendons, ligaments, and capsules.Using the immunohistological method it was possible to identify specific collagen types in areas undergoing rapid proliferation and collagen transition, such as diaphyseal and epiphyseal perichondrium, or in enchondral osteogenesis. During enchondral ossification type I collagen is deposited onto the eroded surface of cartilage. It partially diffuses into the cartilage matrix forming a “hybrid” collagen matrix with type II collagen, which is a site for subsequent ossification. During appositional growth of diaphyseal cartilage and differentiation of epiphyseal perichondrium into articular cartilage, perichondral cells switch from type I to type II collagen synthesis when differentiating into chondroblasts. In the transition zones, chondroblasts are imbedded in a “hybrid” matrix consisting of a mixture of type I and type II collagens.