Regulation of hepatic estrogen receptor isoform mRNA expression in rainbow trout (Oncorhynchus mykiss)
Regulation of hepatic estrogen receptor isoform mRNA expression in rainbow trout (Oncorhynchus mykiss)
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DOI：
10.1016/j.ygcen.2008.11.022
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发表时间：
2009-03-01
影响因子：
2.7
通讯作者：
Cloud, J. G.
中科院分区：
文献类型：
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作者：
Boyce-Derricott, Josh;Nagler, James J.;Cloud, J. G.
虹鳟鱼中完整的核雌激素受体家族由两种亚型（ER α 和 ER β）组成，每种亚型由两种亚型（α 1/α 2 和 β 1/β 2）组成。 ER α 1 的转录速率和 mRNA 稳定性受 17 β-雌二醇 (E2) 影响，但没有关于其他亚型的雌激素调节的信息。本研究的目的是使用定量 RT-PCR 比较雄性鳟鱼肝脏和用 E2 或 17 α-乙炔雌二醇 (EE2) 处理的永生化鳟鱼肝细胞系（RTH-149 和 SOB-15）中四种 ER 亚型的 mRNA 表达模式。为了确定体内剂量反应，将等基因雄性鳟鱼腹膜内注射0、1.5、15或150μg E2或等摩尔量的EE2，并在24小时后对肝脏取样。 E2 或 EE2 治疗显着 (p
The complete nuclear estrogen receptor family in rainbow trout consists of two subtypes (ER alpha and ER beta) each of which consists of two isoforms (alpha 1/alpha 2 and beta 1/beta 2). Transcription rate and mRNA stability of ER alpha 1 is affected by 17 beta-estradiol (E2) but no information on estrogen regulation exists for the other isoforms. The objective of this study was to compare the mRNA expression patterns of the four ER isoforms in the liver of male trout and in immortalized trout hepatocyte lines (RTH-149 and SOB-15) treated with E2 or 17 alpha-ethynylestradiol (EE2) using quantitative RT-PCR. To determine the in vivo dose-response, isogenic male trout were injected intra-peritoneally with 0, 1.5, 15 or 150 mu g E2 or an equimolar amount of EE2 and the liver sampled 24 h later. Treatment with either E2 or EE2 significantly (p