Sentinel Lymph Nodes in the Rat: Noninvasive Photoacoustic and US Imaging with a Clinical US System
Sentinel Lymph Nodes in the Rat: Noninvasive Photoacoustic and US Imaging with a Clinical US System
复制标题
DOI：
10.1148/radiol.10091772
复制
发表时间：
2010-07-01
期刊：
影响因子：
19.7
通讯作者：
Wang, Lihong V.
中科院分区：
文献类型：
--
作者：
Erpelding, Todd N.;Kim, Chulhong;Wang, Lihong V.
目的：评价在体内前哨淋巴结（SLN）映射使用光声和超声（US）成像与改进的临床US imaging systems.Materials和方法：动物方案批准的动物研究委员会。通过使用从临床US成像系统改编的光声成像系统对7只健康Sprague-Dawley大鼠腋窝淋巴结中的亚甲蓝染料积聚进行成像。为了研究临床转化，通过在大鼠皮肤表面上添加鸡或火鸡胸脯肉，将成像深度扩展至2.5 cm。三维光声图像通过机械扫描的US换能器和光传输纤维束沿着elevation direction.Results：光声图像的大鼠前哨淋巴结清楚地帮助可视化的亚甲蓝积累，而共配准的光声/US图像描绘淋巴结的位置相对于周围的解剖结构。亚甲蓝注射后20分钟，SLN区域的光声信号从注射前图像的基线信号增加了近33倍，SLN和背景组织之间的平均对比度为76.0 +/- 23.7（标准差）。亚甲蓝在前哨淋巴结中的积累被证实通过使用染料的光吸收光谱的光声。三维光声图像显示的动态积累的亚甲蓝在SLNs后，通过lymph vessels.Conclusion：在体内光声和超声映射的SLNs成功地证明了一个改进的临床US扫描仪。这些结果提高了光声和超声成像在临床上可用于乳腺癌患者腋窝淋巴结分期的前哨淋巴结的准确，非侵入性成像的信心。(C)RSNA，2010年
Purpose: To evaluate in vivo sentinel lymph node (SLN) mapping by using photoacoustic and ultrasonographic (US) imaging with a modified clinical US imaging system.Materials and Methods: Animal protocols were approved by the Animal Studies Committee. Methylene blue dye accumulation in axillary lymph nodes of seven healthy Sprague-Dawley rats was imaged by using a photoacoustic imaging system adapted from a clinical US imaging system. To investigate clinical translation, the imaging depth was extended up to 2.5 cm by adding chicken or turkey breast on top of the rat skin surface. Three-dimensional photoacoustic images were acquired by mechanically scanning the US transducer and light delivery fiber bundle along the elevational direction.Results: Photoacoustic images of rat SLNs clearly help visualization of methylene blue accumulation, whereas coregistered photoacoustic/US images depict lymph node positions relative to surrounding anatomy. Twenty minutes following methylene blue injection, photoacoustic signals from SLN regions increased nearly 33-fold from baseline signals in preinjection images, and mean contrast between SLNs and background tissue was 76.0 +/- 23.7 (standard deviation). Methylene blue accumulation in SLNs was confirmed photoacoustically by using the optical absorption spectrum of the dye. Three-dimensional photoacoustic images demonstrate dynamic accumulation of methylene blue in SLNs after traveling through lymph vessels.Conclusion: In vivo photoacoustic and US mapping of SLNs was successfully demonstrated with a modified clinical US scanner. These results raise confidence that photoacoustic and US imaging can be used clinically for accurate, noninvasive imaging of SLNs for axillary lymph node staging in breast cancer patients. (C) RSNA, 2010