High-throughput determination of thioglycolic acid lignin from rice
High-throughput determination of thioglycolic acid lignin from rice
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DOI：
10.5511/plantbiotechnology.26.337
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发表时间：
2009-01-01
影响因子：
1.6
通讯作者：
Umezawa, Toshiaki
中科院分区：
文献类型：
--
作者：
Suzuki, Shiro;Suzuki, Yoshie;Umezawa, Toshiaki
为了研究和开发适合饲料和生物燃料生产的植物，开发高通量木质素测定系统，特别是草本植物，是很重要的。然而，现有的木质素测定方法并不适用于草本样品的高通量分析。在本文中，我们描述了一种简单、高通量的方法，用于测定稻秆中巯基乙酸木质素，该方法使用一次性塑料微管、TissueLyser和紫外微孔板阅读器。我们以竹磨木质素制备的巯基乙酸木质素为木质素标准，成功绘制了由分光光度计和微孔板阅读器读数得出的校准曲线。根据标定曲线，可以测定稻秆和稻苗各器官中木质素的含量。我们证实，20毫克的干燥材料从水稻植株足以稳定测定木质素含量。使用这种方法，每个人在三天内对100个独立的稻草样本进行分析。
For research and development of plants suitable for forage and biofuel production, the development of a high-throughput system of lignin determination, especially for herbaceous species, is important. However, currently available methods for lignin determination are not Suitable for high-throughput analysis of herbaceous samples. In this paper, We describe a straightforward, high-throughput method for lignin determination as thioglycolic acid lignin from rice straw using disposable plastic microtubes, TissueLyser, and a UV microplate reader. We successfully plotted calibration curves derived from the readings of a spectrophotometer and a microplate reader using thioglycolic acid lignin prepared from bamboo milled wood lignin as a lignin standard. Based oil the calibration Curve, we could determine the lignin content in various organs from dried rice straw and rice seedlings. We confirmed that 20 mg of dried material From rice plants is adequate for stable determination of the lignin content. Using this method, 100 independent samples from rice straw call be analyzed in three days per person.