Cardiomyocyte transplantation does not reverse cardiac remodeling in rats with chronic myocardial infarction
Cardiomyocyte transplantation does not reverse cardiac remodeling in rats with chronic myocardial infarction
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DOI：
10.1016/s0003-4975(02)03601-9
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发表时间：
2002-07-01
影响因子：
4.6
通讯作者：
Komeda, M
中科院分区：
文献类型：
--
作者：
Sakakibara, Y;Tambara, K;Komeda, M
背景一些报告已经记录了细胞移植作为心脏移植的替代方案的潜在益处。本研究旨在探讨心肌细胞移植对慢性心肌梗死大鼠的治疗作用。本研究使用同系刘易斯大鼠。结扎大鼠左前降支动脉诱发慢性心肌梗死。4周后，超声心动图证实左心室（LV）功能障碍伴无运动区域后，将大鼠随机分为两组：接受胎儿心肌细胞移植组（TX组; n = 11）和仅接受心肌内注射培养基组（对照组; n = 12）。治疗4周后，TX组的收缩末期内径（LVDs）（7.5 ± 0.9 vs 8.9 ± 0.8 mm，p < 0.01）和短轴缩短率（FS）（26.2 ± 5.9 vs 17.7% ± 5.1%，p < 0.01）均小于对照组。然而，TX组的基线和治疗后值之间的LV舒张末期尺寸、LVD和FS无差异。此外，治疗4周后两组血浆心钠素水平无显著差异。在显微镜下观察，TX组仅在梗死周边区发现少量移植心肌细胞，而在瘢痕区中心未发现移植心肌细胞，梗死区室壁增厚。胚胎心肌细胞移植可预防但不能逆转心肌梗死大鼠伴随心力衰竭的心脏重构。进一步的研究是必要的最佳临床应用于衰竭的心脏。(C)2002年由胸外科医师协会出版。
Background. Several reports have documented the potential benefits of cell transplantation as an alternative to cardiac transplantation. This study was designed to investigate whether cardiomyocyte transplantation is effective in rats with chronic myocardial infarction.Methods. Syngeneic Lewis rats were used in this study. Chronic myocardial infarction was induced in rats by ligating the left anterior descending artery. Four weeks later, after left ventricular (LV) dysfunction with akinetic regions was confirmed by echocardiography, the rats were randomized into two groups: a group that received fetal cardiomyocyte transplantation (TX group; n = 11); and a group that received an intramyocardial injection of culture medium only (control group; n = 12).Results. Four weeks after treatment, the TX group had smaller end-systolic dimension (LVDs) (7.5 +/- 0.9 vs 8.9 +/- 0.8 mm, p < 0.01) and better fractional shortening (FS) (26.2 +/- 5.9 vs 17.7% +/- 5.1%, p < 0.01) than the control group. However, there were no differences in LV end-diastolic dimension, LVDs, and FS between baseline and post-treatment values in the TX group. In addition, plasma levels of atrial natriuretic peptide were not significantly different between the two groups 4 weeks after treatment. In microscopic examination, small amounts of transplanted cardiomyocytes were found only in the periinfarct area, not in the center of scar area, and a thicker ventricular wall in the infract area was detected in the TX group.Conclusions. Fetal cardiomyocyte transplantation prevented, but did not reverse, cardiac remodeling that was accompanied with heart failure in myocardial infarction rats. Further investigation is warranted for optimal clinical application to the failing heart. (C) 2002 by The Society of Thoracic Surgeons.