Identification of genetically aberrant cell lineages in Wilms' tumors.
Identification of genetically aberrant cell lineages in Wilms' tumors.
复制标题
肾母细胞瘤中遗传异常细胞谱系的鉴定。
DOI：
10.1002/gcc.2870100107
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Fletcher,JA
中科院分区：
文献类型：
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作者：
Weremowicz,S;Kozakewich,HP;Haber,D;Park,S;Morton,CC;Fletcher,JA
大多数肾母细胞瘤含有几种主要的细胞类型，其中原始芽基群体通常是最突出的。其他典型的成分包括未分化的间充质细胞和上皮细胞，但尚未证明这些成分是肿瘤性的。我们采用细胞遗传学和荧光原位杂交相结合的方法来确定6个肾母细胞瘤内不同细胞群的克隆关系。3例肾母细胞瘤的染色体数目畸变均发生在胚细胞中，而间充质细胞中未见。在胚基细胞和间充质细胞中检测到另外两个肿瘤中一个WT 1等位基因的缺失。在第六例中，在间充质和上皮细胞中观察到18号四体;在该肿瘤中无法评价胚细胞。这些发现表明，间充质和上皮细胞在一些肾母细胞瘤是肿瘤。在某些肾母细胞瘤中，不同的组织学成分来源于一个染色体异常的祖先，这很可能是原始胚基细胞。Genes Chrom Cancer 10：40-48（1994）。© 1994 Wiley利斯公司
Most Wilms' tumors contain several predominant cell types, of which a primitive blastemal population is often the most prominent. Other typical components include undifferentiated mesenchymal and epithelial cells, but it has not been demonstrated that these components are neoplastic. We used a combined cytogenetic and fluorescence in situ hybridization approach to determine the clonal relationship of different cell populations within six Wilms' tumors. Clonal numerical chromosome aberrations in three Wilms' tumors were found in blastemal cells, but not in mesenchymal cells. Loss of one WTl allele in two other tumors was detected in both blastemal and mesenchymal cells. Tetrasomy 18 in a sixth case was observed in mesenchymal and epithelial cells; blastemal cells could not be evaluated in this tumor. These findings demonstrate that mesenchymal and epithelial cells in some Wilms' tumors are neoplastic. Different histologic components in some Wilms' tumors derive from a single chromosomally aberrant ancestor which is most likely to be the primitive blastemal cell. Genes Chrom Cancer 10:40–48 (1994). © 1994 Wiley‐Liss, Inc.