Development of new inbred transgenic strains of rats with LacZ or GFP
Development of new inbred transgenic strains of rats with LacZ or GFP
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DOI：
10.1016/j.bbrc.2005.01.132
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发表时间：
2005-04-01
影响因子：
3.1
通讯作者：
Kobayashi, E
中科院分区：
文献类型：
--
作者：
Inoue, H;Ohsawa, I;Kobayashi, E
再生医学的理想目标是恢复受损组织的形式和功能。虽然干细胞移植被认为是一种有前途的治疗方法，但使用适当的标记物了解移植细胞的命运至关重要。我们在转基因（Tg）动物技术的基础上，建立了新的LacZ和GFP近交系转基因大鼠品系。与之前的Tg大鼠相比，这些Tg动物普遍表达了大部分标记基因。使用常规皮肤移植评估针对标记蛋白的免疫抗原性，结果表明lacZ-Tg衍生的皮肤的免疫原性比GFP-Tg低得多。然而，来自亲本转基因大鼠的GFP阳性细胞仍然是研究免疫赦免部位（如大脑）细胞命运的潜在候选者。利用免疫原性较低的lacZ，我们还研究了骨髓来源的细胞（BMDCs）在皮肤伤口愈合中的作用，使用体内生物成像系统。虽然BMDC移植增强了注射部位的伤口愈合，但仅在短时间内检测到BMDC，表明自体BMDC移植对伤口愈合的短暂贡献。我们的TG-大鼠系统可能为再生医学的细胞过程的阐明提供巨大的益处，包括细胞和组织移植。(C)2005年爱思唯尔公司All rights reserved.
The ideal goal of regeneration medicine is to restore form and function to damaged tissues. While stem cell transplantation is considered a promising therapeutic approach, knowing the fate of transplanted cells using appropriate markers is essential. We developed new inbred transgenic rat strains with lacZ and GFP based on the transgenic (Tg) animal technique in rats. These Tg animals expressed most of their marker genes ubiquitously, compared to previous Tg rats. Immunological antigenicity against marker proteins was evaluated using conventional skin grafting, and results suggested lacZ-Tg-derived skin was much less immunogenic than that of GFP-Tg. However, GFP-positive cells from parental transgenic rats were still potential candidates for the study of cellular fate in immune privilege sites, such as the brain. Taking advantage of less immunogenic lacZ, we also examined the role of bone marrow-derived cells (BMDCs) in skin wound healing using an in vivo biological imaging system. Although transplantation of BMDCs enhanced wound healing at the injection site, BMDCs were detected only for a short time, suggesting a transient contribution of autologous BMDC-transplantation in wound healing. Our Tg-rat system may provide great benefits for the elucidation of the cellular process of regenerative medicine, including cell and tissue transplantation. (C) 2005 Elsevier Inc. All rights reserved.