Lack of association between Helicobacter sp colonization and gallstone disease
Lack of association between Helicobacter sp colonization and gallstone disease
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DOI：
10.1097/00004836-200102000-00009
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发表时间：
2001-02-01
影响因子：
2.9
通讯作者：
Uribe, M
中科院分区：
文献类型：
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作者：
Méndez-Sánchez, N;Pichardo, R;Uribe, M
最近，在智利慢性胆囊炎患者的切除胆囊组织和收集的胆汁中发现了螺杆菌。因此，有人提出胆汁螺杆菌与胆囊癌之间存在关联。有趣的是，螺杆菌定植和胆结石病（GD）在智利非常频繁地发生。然而，螺杆菌定植与 GD 之间是否存在关联尚未得到充分研究。本研究的目的是确定患有 GD 的人类胆囊组织中螺杆菌的发病率。该研究纳入了 95 名接受胆囊切除术的墨西哥患者。使用螺杆菌特异性 16-S 核糖体 RNA 引物，使用组织学、免疫组织化学和聚合酶链反应 (PCR) 分析对收集的胆囊标本进行评估，以鉴定螺杆菌。详细检查的 95 例标本中，均存在结石，具体情况如下： 56 例（59%）患有慢性胆囊炎； 7例（7.4%），急性胆囊炎：15例（16%）。慢性和急性胆囊炎，10 例（9.5%）胆固醇中毒，7 例（7.4%）淋巴组织增生。当组织学呈阳性时，标本被认为对螺杆菌呈阳性。通过免疫组织化学分析，95 份标本中只有 1 份呈螺杆菌阳性，即 32 份标本中有 1 份呈螺杆菌阳性。通过PCR。这些结果表明墨西哥 GD 患者胆囊上皮螺杆菌的发生率较低。然而。我们不能忽视胆囊上皮中不常见的螺杆菌的存在及其与胆结石发病机制的关系。此外，这项研究表明墨西哥人群中 GD 与螺杆菌定植之间没有明显关联。
Recently, Helicobacter sp has been identified in resected gallbladder tissue and in collected bile from Chilean patients with chronic cholecystitis. Therefore, it an association between bile Helicobacter sp and gallbladder cancer has been proposed. Interestingly, both Helicobacter colonization and gallstone disease (GD) happen very frequently in Chile. However, whether there is an association between Helicobacter colonization and GD has not been completely studied. The: aim of this study was to determine the incidence of Helicobacter in human gallbladder tissues with GD. The study included 95 Mexican patients undergoing cholecystectomy. Collected gallbladder specimens were assessed to identify Helicobacter sp using histology, immunohistochemistry, and polymerase chain reaction (PCR) analysis using Helicobacter-specific 16-S ribosomal RNA primers. Of the 95 specimens examined in detail, all had stones as follows: 56 (59%) had chronic cholecystitis; 7 (7.4%), acute cholecystitis: 15 (16%). both chronic and acute cholecystitis, 10 (9.5%), cholesterolosis, and 7 (7.4%), lymphoid hyperplasia. Specimens were considered positive for Helicobacter when histology was positive. Only 1 of the 95 specimens was positive for Helicobacter by immunohistochemistry analysis, 1 of 32 cases. by PCR. These results suggest a low incidence of Helicobacter the gallbladder epithelium of Mexican patients with GD. However. we can not discard the existence of uncommon Helicobacter sp in gallbladder epithelium and its association with gallstone pathogenesis. Additionally, this study suggests no apparent association between GD and Helicobacter colonization in a Mexican population.