Identification of flagellar proteins that initiate the activation of sperm motility in vivo
Identification of flagellar proteins that initiate the activation of sperm motility in vivo
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DOI：
10.1006/bbrc.1997.7937
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发表时间：
1998-01-06
影响因子：
3.1
通讯作者：
Tash, JS
中科院分区：
文献类型：
--
作者：
Bracho, GE;Fritch, JJ;Tash, JS
蛋白质磷酸化似乎是启动精子活力激活的细胞内信号通路中的必要步骤。活的不动的海胆精子的激活产生快速的，时间依赖性增加磷酸化的蛋白质的32，45，130，和500 kDa。不动和能动精子的分馏表明，这些运动相关的磷蛋白与鞭毛。这些蛋白质在来自活动精子的鞭毛部分中显示出更大的磷酸化，表明亚细胞边界是适当的，以保持蛋白激酶和它们的底物在空间上分离。溶解度性质表明这些蛋白质是海胆精子动力蛋白的重链和更小的亚基，其在体内被磷酸化以启动运动性的激活。这也表明仅这几种蛋白质的磷酸化，在已知发生在基本轴丝中的近100种磷酸化中，有一种磷酸化似乎与完整精子中运动性激活的早期信号通路有关。(C)北京：科学出版社.
Protein phosphorylation appears to be a necessary step in the intracellular signaling pathway that initiates the activation of sperm motility. Activation of live immotile sea urchin sperm produced rapid, time-dependent increased phosphorylation on proteins of 32, 45, 130, and 500 kDa. Fractionation of immotile and motile sperm indicated that these motility-related phosphoproteins are associated with flagella. These proteins showed greater phosphorylation in the flagellar fraction from motile sperm, suggesting that subcellular boundaries are in place to keep protein kinases and their substrates spatially separated, Solubility properties suggest that these proteins are the heavy chain and smaller subunits of sea urchin sperm dynein which are phosphorylated in vivo to initiate activation of motility, This also suggests that phosphorylation of only these few proteins, out of the nearly 100 phosphorylations known to occur in the basic axoneme, appears to be associated with the early signaling pathways of motility activation in intact sperm. (C) 1998 Academic Press.