IR-783 Labeling of a Peptide Receptor for 'Turn-On' Fluorescence Based Sensing.
IR-783 Labeling of a Peptide Receptor for 'Turn-On' Fluorescence Based Sensing.
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DOI：
10.3390/chemosensors6040047
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发表时间：
2018-10
期刊：
影响因子：
4.2
通讯作者：
Eun-A Kwak;LeNaiya A. Kydd;Butaek Lim;J. Jaworski
中科院分区：
文献类型：
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作者：
Eun-A Kwak;LeNaiya A. Kydd;Butaek Lim;J. Jaworski
在这项研究中，我们研究了一种开发近红外荧光传感器的方法，通过流线型的、位点特异性的与基于多肽的受体的耦合。作为固相合成多肽受体的倒数第二步，我们展示了一种用近红外荧光团IR-783标记N‘端的简单方法，以提供从树脂中裂解后的可行的荧光传感器。概念验证探针利用生物素模拟肽序列作为接受部分。在这里，我们揭示了当生物素模拟探针与其预期的链霉亲和素目标结合时，“开启”的荧光增强。并不是所有被测试的多肽受体部分都能够在靶标结合时产生这样的增强，因此，我们讨论了观察到的荧光反应特性的基本原理。
In this study, we examine a means for developing near-IR fluorescent sensors through streamlined, site-specific coupling with peptide-based receptors. As the penultimate step of solid-phase synthesis of a peptide-based receptor, we show a simple means of labeling the N' terminus with the near IR fluorophore IR-783 to afford a viable fluorescent sensor after cleavage from the resin. The proof-of-concept probe utilized a biotin mimetic peptide sequence as the receptive moiety. Here we revealed a "turn-on" fluorescence enhancement upon binding of the biotin mimetic probe to its intended streptavidin target. Not all peptide-receptive moieties tested were able to generate such an enhancement upon target binding, and as such, the rationale for the observed fluorescence response properties is discussed.