Cloning and expression analysis of rainbow trout Oncorhynchus mykiss interferon regulatory factor 1 and 2 (IRF-1 and IRF-2)
Cloning and expression analysis of rainbow trout Oncorhynchus mykiss interferon regulatory factor 1 and 2 (IRF-1 and IRF-2)
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DOI：
10.1016/s0145-305x(02)00072-1
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发表时间：
2003-02-01
影响因子：
2.9
通讯作者：
Secombes, CJ
中科院分区：
文献类型：
--
作者：
Collet, B;Hovens, GCJ;Secombes, CJ
干扰素调节因子 (IRF) I 和 2 的两个虹鳟鱼 (Oncorhynchus mykiss) 基因已被克隆并测序。 IRF-1 cDNA 包含 996 个核苷酸的开放阅读框 (ORF)，可翻译为 331 个氨基酸的推定肽，具有 145 bp 的 5' 非翻译区 (UTR) 和 481 bp 的 3' UTR。 IRF-2 cDNA 包含一个 1035 bp 的 ORF，可翻译为 344 个氨基酸的推定肽，其中 5' UTR 为 146 bp，3' UTR 为 925 bp。在体内，IRF-1和IRF-2在头肾、鳃和脾中组成型表达，但在肝脏中不表达。这两种基因在所有检查的组织中均被诱导。在针对病毒性出血性败血症病毒进行DNA疫苗接种一周后，注射部位的IRF-1表达显着增加，但IRF-2表达没有显着增加。在体外，IRF-1 和 IRF-2 转录物存在于未刺激的虹鳟性腺细胞中，并被 Poly I/C 上调。 (C) 2002 Elsevier Science Ltd. 保留所有权利。
Two rainbow trout (Oncorhynchus mykiss) genes for interferon regulatory factors (IRF) I and 2 have been cloned and sequenced. The IRF-1 cDNA contains an open reading frame (ORF) of 996 nucleotides that translates into a 331 amino-acid putative peptide, with a 5' untranslated region (UTR) of 145 bp and a 3' UTR of 481 bp. The IRF-2 cDNA contains a 1035 bp ORF that translates into a 344 amino-acid putative peptide, with a 5' UTR of 146 bp and a 3' UTR of 925 bp. In vivo, IRF-1 and IRF-2 are constitutively expressed in head kidney, gill and spleen but not liver. Both genes were induced in all the tissues examined. IRF-1 but not IRF-2 expression was significantly increased at the site of injection I week after DNA vaccination against viral haemorrhagic septicaemia virus. In vitro, IRF-1 and IRF-2 transcripts are present in unstimulated rainbow trout gonad cells and are up-regulated by poly I/C. (C) 2002 Elsevier Science Ltd. All rights reserved.