Identification and characterization of a LEA family gene CarLEA4 from chickpea (Cicer arietinum L.)
Identification and characterization of a LEA family gene CarLEA4 from chickpea (Cicer arietinum L.)
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鹰嘴豆 (Cicer arietinum L.) 中 LEA 家族基因 CarLEA4 的鉴定和表征
DOI：
10.1007/s11033-011-1130-6
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发表时间：
2012
影响因子：
2.8
通讯作者：
Hao Ma
中科院分区：
文献类型：
--
作者：
H. Gu;Yuying Jia;Xiansheng Wang;Quanjia Chen;Shu;Lin Ma;Jusong Zhang;Hua Zhang;Hao Ma
胚胎发育晚期丰度（LEA）蛋白与种子发育过程中耐干燥性的获得以及植物对干旱、盐、冰冻等的反应密切相关。在本研究中，一个LEA基因CarLEA4 (GenBank登录号：利用聚乙二醇（PEG）处理鹰嘴豆幼苗叶片构建的cDNA文库，从鹰嘴豆中分离到GU247511)。CarLEA4在基因组DNA序列中含有2个外显子和1个内含子，编码了152个氨基酸的多肽。CarLEA4具有保守的pfam结构域，二级结构与第4组LEA蛋白高度相似。它局限于细胞核。CarLEA4转录本在鹰嘴豆幼苗叶、茎、根、花、幼荚和幼种子等多个器官中均检测到。CarLEA4受叶龄的抑制，在种子发育、荚果发育和萌发过程中表达发生变化。此外，干旱、盐、热、冷、ABA、IAA、GA3和MeJA对CarLEA4的表达有强烈的诱导作用。我们的研究结果表明，CarLEA4编码LEA组4的一个蛋白，可能参与了植物的多种发育过程和非生物胁迫反应。
Late-embryogenesis abundant (LEA) proteins have been reported to be closely correlated with the acquisition of desiccation tolerance during seed development and response of plant to drought, salinity, and freezing, etc. In this study, a LEA gene, CarLEA4 (GenBank accession no. GU247511), was isolated from chickpea based on a cDNA library constructed with chickpea seedling leaves treated by polyethylene glycol (PEG). CarLEA4 contained two exons and one intron within genomic DNA sequence and encoded a putative polypeptide of 152 amino acids. CarLEA4 had a conserved pfam domain, and showed high similarity to the group 4 LEA proteins in secondary structure. It was localized in the nucleus. The transcripts of CarLEA4 were detected in many chickpea organs including seedling leaves, stems, roots, flowers, young pods, and young seeds. CarLEA4 was inhibited by leaf age and showed expression changes in expression during seed development, pod development and germination. Furthermore, the expression of CarLEA4 was strongly induced by drought, salt, heat, cold, ABA, IAA, GA3 and MeJA. Our results suggest that CarLEA4 encodes a protein of LEA group 4 and may be involved in various plant developmental processes and abiotic stress responses.