A Comparative Study in the Printability of a Bioink and 3D Models Across Two Bioprinting Platforms.
A Comparative Study in the Printability of a Bioink and 3D Models Across Two Bioprinting Platforms.
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两个生物打印平台的生物墨水和 3D 模型的可打印性比较研究。
DOI：
10.1016/j.matlet.2020.127382
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发表时间：
2020
影响因子：
3
通讯作者：
Joddar,Binata
中科院分区：
文献类型：
--
作者：
Alonzo,Matthew;Dominguez,Erick;Alvarez-Primo,Fabian;Quinonez,Amado;Munoz,Erik;Puebla,Jazmin;Barron,Antonio;Aguirre,Luis;Vargas,Ana;Ramirez,JeanM;Joddar,Binata
在这项研究中，我们使用藻酸盐-明胶生物墨水来设计和打印具有晶格、蜂窝和纤维束图案的3D结构。这些设计最初是使用小型实验室打印机打印的，后来翻译成更大规模、高吞吐量的打印平台。通过大体形态评价、扫描电子显微镜和肿胀试验对两种不同平台打印的结构进行比较分析，证实了我们的假设，即使用小型实验室生物打印机打印的设计可以成功地转化为大规模打印平台，用于高通量打印构建体。由于打印设计是使用两台打印机通用的软件实现的，因此这个端点是可行的。打印参数的唯一差异是挤出压力的变化，这是由于两台打印机使用的滚筒尺寸有很大差异(3毫升对30毫升)，而所有其他参数保持不变。尽管支架没有被细胞生物打印，但在未来，我们将研究如何通过两个平台上挤出压力的变化来不同地调节细胞活性。
In this study, we used an alginate-gelatin bioink to design and print 3D constructs with lattice, honeycomb and fibrous bundle patterns. These designs were printed using a small-scale laboratory printer at first, and later translated to a larger scale, high throughput-printing platform. A comparative analysis of the structures printed using two dissimilar platforms using gross morphologic evaluation, scanning electron microscopy and swelling assay confirmed our hypothesis that a design printed using a small-scale laboratory bioprinter for optimization of bioink composition and printing parameters can be successfully translated into a large scale-printing platform for high throughput printing of constructs. Since the designs for printing were implemented using a software which was common across both printers, this endpoint was feasible. The only difference in printing parameters resulted from variation in extrusion pressure which was due to a significant difference in barrel size used across both printers (3 ml versus 30 ml), while all other parameters stayed the same. Although the scaffolds were not bioprinted with cells, in future we will investigate how cell viability can be differentially regulated by the variation of extrusion pressure across both platforms.