Comparative analysis of the humoral immunity of skin mucus from several marine teleost fish
Comparative analysis of the humoral immunity of skin mucus from several marine teleost fish
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DOI：
10.1016/j.fsi.2014.06.018
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发表时间：
2014-09-01
影响因子：
4.7
通讯作者：
Esteban, Maria A.
中科院分区：
文献类型：
--
作者：
Guardiola, Francisco A.;Cuesta, Alberto;Esteban, Maria A.
鱼皮肤粘液含有几种免疫物质，它们提供了抵御广泛病原体的第一道防线，尽管迄今为止对它们的研究很少。测定了5种海洋硬骨鱼皮肤粘液中的末端碳水化合物组成、总IgM抗体、几种免疫相关酶(溶菌酶、过氧化物酶、碱性磷酸酶、酯酶、蛋白水解酶和抗蛋白水解酶)以及杀菌活性(对鱼类致病弧菌哈氏弧菌、血管内弧菌、丹姆光杆菌和非致病菌大肠杆菌、枯草芽孢杆菌、腐烂希万氏杆菌)。首先，凝集素结合结果表明，皮肤粘液按丰度顺序含有N-乙酰神经氨酸、葡萄糖、N-乙酰氨基葡萄糖、N-乙酰半乳糖胺、半乳糖和岩藻糖残基。其次，结果表明，虽然一些免疫活性(如IgM和溶菌酶活性)在所研究的鱼类中非常相似，但其他一些活性，如蛋白酶、抗蛋白酶、碱性磷酸酶、酯酶和过氧化物酶活性因鱼种而异。圆盘藻的过氧化物酶活性和蛋白水解酶活性均高于其他物种，而边缘毛藻和金鱼的碱性磷酸酶和酯酶活性分别最高。此外，金黄色葡萄球菌的皮肤粘液对病原菌具有较高的杀菌活性，而对非病原菌(大肠杆菌、枯草杆菌)的杀菌活性则相反。因此，研究鱼皮肤粘液的碳水化合物分布和免疫相关成分的变化有助于了解鱼的抵抗力以及病原体的存在和分布以及感染的程度，这些方面对水产养殖业非常重要。(C)2014爱思唯尔有限公司。保留所有权利。
Fish skin mucus contains several immune substances that provide the first line of defence against a broad spectrum of pathogens although they are poorly studied to date. Terminal carbohydrate composition and levels of total IgM antibodies, several immune-related enzymes (lysozyme, peroxidase, alkaline phosphatase, esterases, proteases and antiproteases) as well as the bactericidal activity (against fish pathogenic Vibrio harveyi, Vibrio angillarum, Photobacterium damselae and non-pathogenic bacteria Escherichia coli, Bacillus subtilis, Shewanella putrefaciens) were identified and measured in the skin mucus of five marine teleosts: gilthead seabream (Sparus aurata), European sea bass (Dicentrarchus labrax), shi drum (Umbrina cirrosa), common dentex (Dentex dentex) and dusky grouper (Epinephelus marginatus). First, lectin binding results suggests that skin mucus contain, in order of abundance, N-acetylneuraminic acid, glucose, N-acetyl-glucosamine, N-acetyl-galactosamine, galactose and fucose residues. Second, results showed that while some immune activities were very similar in the studied fish (e.g. IgM and lysozyme activity) other such as protease, antiprotease, alkaline phosphatase, esterase and peroxidase activities varied depending on the fish species. High levels of peroxidase and protease activity were found in U. cirrosa respect to the values obtained in the other species while E. marginatus and S. aurata showed the highest levels of alkaline phosphatase and esterase activities, respectively. Moreover, skin mucus of S. aurata revealed higher bactericidal activity against pathogenic bacteria, contrarily, to what happened with non-pathogenic bacteria (E. coli, B. subtilis). Thus, study of the variations in the carbohydrate profile and immune-related components of the fish skin mucus could help to understand the fish resistance as well as the presence and distribution of pathogens and magnitude of infections, aspects that are of major importance for the aquaculture industry. (c) 2014 Elsevier Ltd. All rights reserved.