Detection of African genotype in Hyalomma tick pools during Crimean Congo hemorrhagic fever outbreak, Rajasthan, India, 2019
Detection of African genotype in Hyalomma tick pools during Crimean Congo hemorrhagic fever outbreak, Rajasthan, India, 2019
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DOI：
10.1016/j.virusres.2020.198046
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发表时间：
2020-09-01
期刊：
影响因子：
5
通讯作者：
Gopalakrishnan, Maya
中科院分区：
文献类型：
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作者：
Sahay, Rima R.;Dhandore, Suhas;Gopalakrishnan, Maya
克里米亚刚果出血热（CCHF）是一种人畜共患病毒性疾病，主要表现为发热和出血。自2011年至2019年古吉拉特邦和2014年和2015年拉贾斯坦邦报告了几起CCHF暴发后，本研究报告了自2019年8月至2019年11月在拉贾斯坦邦的焦特布尔、贾沙梅尔和Sirohi三个地区记录的5例CCHF暴发。在家畜样本中记录到高百分比的CCHFV IgG抗体阳性。使用下一代测序方法对CCHF病毒（CCHFV）阳性人血液样本和璃眼蜱合并样本进行测序。在研究中，从蜱池中鉴定了两种不同的编码糖蛋白前体的M片段基因型：第一种来自亚洲，第二种来自非洲谱系。L基因（聚合酶）和S基因（核衣壳）聚集在亚洲血统。本研究说明了存在两种不同的CCHFV谱系正在循环内的Hyalomma蜱池在印度的拉贾斯坦邦。在死亡病例和存活病例之间，M基因的氨基酸序列发生了3.56%的变化。这份报告还为加强印度拉贾斯坦邦其他地区和附近各州的人类、蜱虫和牲畜监测敲响了警钟。生物安全措施、屏障护理沿着以及个人防护设备和利巴韦林药物的可用性将始终是预防医院感染的支柱，以进行适当的病例管理。
Crimean Congo hemorrhagic fever (CCHF) is a zoonotic viral disease presenting with fever and hemorrhagic manifestations in humans. After several outbreaks of CCHF being reported from Gujarat since 2011 till 2019 and from Rajasthan in 2014 and 2015, the present study reports the CCHF outbreak which was recorded from five human cases in three districts Jodhpur, Jaisalmer, and Sirohi of Rajasthan state since August 2019 till November 2019. A high percent of positivity was recorded in livestock animal samples for the CCHFV IgG antibody. CCHF virus (CCHFV) positive human blood samples and Hyalomma tick pool samples were sequenced using next generation sequencing method. Two different M segment genotypes, encoding glycoprotein precursor, were identified from tick pools in the study: first from Asian and second from African lineage. The L gene (polymerase) and the S gene (nucleocapsid) clustered in the Asian lineage. The present study illustrates the existence of two different CCHFV lineages being circulating within the Hyalomma tick pools in the Rajasthan state, India. We also observed 3.56% amino acid changes between the death and the survived case of CCHFV in the M gene. This report also sets an alarm to enhance human, tick and livestock surveillance in other districts of Rajasthan and nearby states of India. Biosafety measures, barrier nursing along with the availability of personal protective equipment and ribavirin drug will always be a mainstay in preventing nosocomial infection for proper case management.