Sequencing, assembly, annotation, and gene expression: novel insights into browning-resistant Luffa cylindrica
Sequencing, assembly, annotation, and gene expression: novel insights into browning-resistant Luffa cylindrica
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DOI：
10.7717/peerj.9661
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发表时间：
2020-08
期刊：
影响因子：
2.7
通讯作者：
Ya-Hui Wang;Xiaohong Liu;Rong-Rong Zhang-Rong;Zhiming Yan;A. Xiong;X. Su
中科院分区：
文献类型：
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作者：
Ya-Hui Wang;Xiaohong Liu;Rong-Rong Zhang-Rong;Zhiming Yan;A. Xiong;X. Su
丝瓜是一种广泛种植于世界温带地区的瓜类作物。布朗宁是影响丝瓜品质的重要因素之一。因此，研究丝瓜布朗宁的分子机理具有重要意义。然而，不同抗褐变能力丝瓜品种的分子多样性还没有得到很好的阐明。在我们的研究中，我们使用高通量测序来确定两个丝瓜品种'2D-2'和'35 D-7'的转录组。共聚类出115，099个单基因，其中22，607个为差异表达基因（DEG）。这些DEG，65编码多酚氧化酶，过氧化物酶，或抗坏血酸过氧化物酶进行了进一步分析。定量实时PCR（RT-qPCR）数据表明LcPPO基因（登录号：Cluster-21832.13892）在“35 D-7”中的表达显著高于“2D-2”。几个POD基因（登录号：簇-21832.19847、簇-21832.30619和簇-48491.2）也上调。对植物TFDB数据库的分析表明，WRKY基因家族等转录因子也可能参与了丝瓜布朗宁的调控。结果表明，与酶促反应相关的基因表达差异可能是导致丝瓜不同品种布朗宁抗性差异的原因之一。本研究为丝瓜抗褐变育种提供了理论依据。
Luffa is a kind of melon crop widely cultivated in temperate regions worldwide. Browning is one of the serious factors affecting the quality of Luffa. Therefore, the molecular mechanism of Luffa browning is of great significance to study. However, the molecular diversity of Luffa cultivars with different browning-resistant abilities has not been well elucidated. In our study, we used high-throughput sequencing to determine the transcriptome of two Luffa cylindrica cultivars ‘2D-2’ and ‘35D-7’. A total of 115,099 unigenes were clustered, of which 22,607 were differentially expression genes (DEGs). Of these DEGs, 65 encoding polyphenol oxidase, peroxidase, or ascorbate peroxidase were further analyzed. The quantitative real-time PCR (RT-qPCR) data indicated that the expression levels of the LcPPO gene (Accession No.: Cluster-21832.13892) was significantly higher in ‘35D-7’ compared with that in ‘2D-2’. Several POD genes (Accession No.: Cluster-21832.19847, Cluster-21832.30619 and Cluster-48491.2) were also upregulated. Analysis of the plantTFDB database indicated that some transcription factors such as WRKY gene family may also participate in the regulation of Luffa browning. The results indicated that the divergence of genes expression related to enzymatic reaction may lead to the different browning resistances of Luffa. Our study will provide a theoretical basis for breeding of browning-resistant Luffa.