A high-throughput Bioreactor system for simulating physiological environments
A high-throughput Bioreactor system for simulating physiological environments
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DOI：
10.1109/tie.2008.928122
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发表时间：
2008-09-01
影响因子：
7.7
通讯作者：
Ahluwalia, Arti
中科院分区：
文献类型：
--
作者：
Mazzei, Daniele;Vozzi, Federico;Ahluwalia, Arti
组织工程、药理学或代谢研究的体外细胞培养的优化需要在不同条件下进行大量实验。在本文中，我们描述了一个高通量的生物反应器系统，允许在一个模拟的类活体环境中进行平行实验。我们的生物反应器由组织、器官或系统特异性培养室和混合装置组成，混合装置由嵌入式系统控制，该系统可调节培养基中的气体插入，以控制pH值和压力。每个培养室和混合装置都拥有一个自主控制系统，能够确保细胞的最佳环境。计算机与嵌入式系统进行通信，以获取数据并设置实验变量。利用该装置，我们可以进行高通量实验，控制多个生物反应器并行工作。在本文中，我们讨论了系统的结构和设计，并提出了一些模拟生理和病理条件下的实验结果。
The optimization of in vitro cell culture for tissue engineering, pharmacological, or metabolic studies requires a large number of experiments to be performed under varying conditions. In this paper, we describe a high-throughput bioreactor system that allows the conduction of parallel experiments in a simulated in vivo-like environment. Our bioreactors consist of tissue-, organ-, or system-specific culture chambers and a mixing device controlled by an embedded system that regulates the insertion of gas in the culture medium in order to control pH and pressure. Each culture chamber and mixing device possesses an autonomous control system that is able to ensure an optimal environment for cells. A computer communicates with the embedded system to acquire data and set up experimental variables. With this apparatus, we can perform a high-throughput experiment controlling several bioreactors working in parallel. In this paper, we discuss the architecture and design of the system, and the results of some experiments which simulate physiological and pathological conditions are presented.