Inhibition of Toxic IAPP Amyloid by Extracts of Common Fruits.
Inhibition of Toxic IAPP Amyloid by Extracts of Common Fruits.
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DOI：
10.1016/j.jff.2014.12.013
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发表时间：
2015-01-01
影响因子：
5.6
通讯作者：
Moffet DA
中科院分区：
文献类型：
--
作者：
Kao PY;Green E;Pereira C;Ekimura S;Juarez D;Whyte T;Arhar T;Malaspina B;Nogaj LA;Moffet DA
37-氨基酸多肽胰岛淀粉样多肽（IAPP，胰淀素）的聚集，作为不溶性淀粉样蛋白或作为小的寡聚体，似乎在2型糖尿病中的胰岛β细胞的死亡中起直接作用。据信，抑制IAPP的聚集可以减缓（如果不能完全防止的话）这种疾病的进展。分析了十三种不同常见水果的提取物防止淀粉样蛋白IAPP聚集的能力。进行硫磺素T结合、免疫检测和圆二色性测定以测试每种提取物的体外抑制潜力。使用原子力显微镜观察有和没有每种水果提取物的淀粉样蛋白原纤维的形成。最后，测试提取物保护活哺乳动物细胞免受淀粉样蛋白IAPP毒性作用的能力。几种水果显示出抑制IAPP聚集和保护活细胞免受有毒IAPP淀粉样蛋白的能力。
The aggregation of the 37-amino acid polypeptide islet amyloid polypeptide (IAPP, amylin), as either insoluble amyloid or as small oligomers, appears to play a direct role in the death of pancreatic β-islet cells in type 2 diabetes. It is believed that inhibiting the aggregation of IAPP may slow down, if not prevent entirely, the progression of this disease. Extracts of thirteen different common fruits were analyzed for their ability to prevent the aggregation of amyloidogenic IAPP. Thioflavin T binding, immuno-detection and circular dichroism assays were performed to test the in vitro inhibitory potential of each extract. Atomic force microscopy was used to visualize the formation of amyloid fibrils with and without each fruit extract. Finally, extracts were tested for their ability to protect living mammalian cells from the toxic effects of amyloid IAPP. Several fruits showed substantial ability to inhibit IAPP aggregation and protect living cells from toxic IAPP amyloid.