miR-185-3p regulates the invasion and metastasis of nasopharyngeal carcinoma by targeting WNT2B in vitro.
miR-185-3p regulates the invasion and metastasis of nasopharyngeal carcinoma by targeting WNT2B in vitro.
复制标题
miR-185-3p通过靶向WNT2B调控鼻咽癌的体外侵袭和转移
DOI：
10.3892/ol.2017.5778
复制
发表时间：
2017-04
期刊：
影响因子：
2.9
通讯作者：
Qiu Y
中科院分区：
文献类型：
--
作者：
Liu C;Li G;Ren S;Su Z;Wang Y;Tian Y;Liu Y;Qiu Y
MicroRNAs(MiRs)已被认为是包括鼻咽癌(NPC)在内的不同类型人类癌症恶性行为的重要调节因子。本课题组先前的一项研究表明miR-185-3p调节鼻咽癌细胞的辐射抗性。本研究旨在探讨miR-185-3p在鼻咽癌侵袭转移中的作用。将miR-185-3p模拟物和miR-185-3p抑制剂分别导入人鼻咽癌CNE-2和5-8F细胞，通过伤口愈合实验和Matrigel侵袭实验观察其对CNE-2和5-8F细胞侵袭转移的影响。为了研究其在鼻咽癌中的作用，利用siRNA技术在5-8F细胞中沉默了miR-185-3p、Wnt家族成员2B(WNT2B)的靶基因。本研究的数据表明，在一系列鼻咽癌细胞系中，miR-185-3p在5-8F细胞中的表达最高，在CNE-2细胞中的表达最低。将miR-185-3p模拟基因导入CNE-2细胞后，伤口愈合和Matrigel侵袭实验表明，与阴性对照组相比，CNE-2细胞的迁移和侵袭能力明显降低。此外，抑制miR-185-3p在5-8F细胞中的迁移和侵袭能力显著增强。此外，沉默WNT2B的表达可显著降低5-8F细胞的侵袭和转移。通过沉默5-8F细胞中的WNT2B，可以逆转抑制miR-185-3p侵袭和转移的作用。本研究结果表明，miR-185-3p在体外通过靶向WNT2B介导鼻咽癌的侵袭和转移。
MicroRNAs (miRs) have been recognised as important regulators of malignant behaviour in different types of human cancer, including nasopharyngeal carcinoma (NPC). A previous study by our group revealed that miR-185-3p regulates the radioresistance of NPC cells. The present study aimed to investigate the effect of miR-185-3p on NPC invasion and metastasis. Human NPC CNE-2 and 5-8F cell lines were transfected with a miR-185-3p mimic and miR-185-3p inhibitor, respectively, and their effects on the invasion and metastasis of these cells was assessed using a wound healing assay and Matrigel invasion assay. The target gene of miR-185-3p, Wnt family member 2B (WNT2B) was silenced in 5-8F cells using siRNA in order to investigate its function in NPC. Data from the present study demonstrated that the expression of miR-185-3p was the highest in 5-8F and lowest in CNE-2 cells out of a range of NPC cell lines. Following the transfection of miR-185-3p mimic into CNE-2 cells, the wound healing and Matrigel invasion assays indicated that the migration and invasion ability of CNE-2 cells was significantly reduced compared with the negative control group. In addition, the inhibition of miR-185-3p in 5-8F cells significantly increased the capacity for migration and invasion. Furthermore, silencing WNT2B expression resulted in a significant reduction in the invasion and metastasis in 5-8F cells. The inhibition of miR-185-3p, which promotes invasion and metastasis, could be reversed through the silencing of WNT2B in 5-8F cells. The results of the present study indicate that miR-185-3p mediates the invasion and metastasis of NPC by targeting WNT2B in vitro.