CHARACTERIZATION OF RETICULOENDOTHELIOSIS VIRUS STRAIN-T DNA AND ISOLATION OF A NOVEL VARIANT OF RETICULOENDOTHELIOSIS VIRUS STRAIN-T BY MOLECULAR-CLONING
CHARACTERIZATION OF RETICULOENDOTHELIOSIS VIRUS STRAIN-T DNA AND ISOLATION OF A NOVEL VARIANT OF RETICULOENDOTHELIOSIS VIRUS STRAIN-T BY MOLECULAR-CLONING
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DOI：
10.1128/jvi.40.3.800-811.1981
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发表时间：
1981-01-01
影响因子：
5.4
通讯作者：
TEMIN, HM
中科院分区：
文献类型：
--
作者：
CHEN, ISY;MAK, TW;TEMIN, HM
网状内皮组织增生症病毒T株(REV-T)是一种高度致癌的禽类逆转录病毒，可引起淋巴网状系统的快速肿瘤性疾病。一旦感染，这种病毒就会产生2个种。未整合的线性病毒DNA，长8.3和5.5千碱基对(KBP)，分别代表辅助病毒(REV-A)和致癌成分(REV-T)。这两个DNA组分的限制性内切酶酶切图谱表明，REV-T DNA相对于REV-A DNA和替换.apprx有很大一部分基因组缺失。0.8-1.5 kbp，与Rev-A DNA无关。这些额外的序列组成了Rev-T(Rel)的假定转换区。用REV-T转化的鸡脾细胞系产生病毒，感染后产生3个种。未整合的线状病毒DNA(8.3、5.5和3.3kbp)。通过克隆噬菌体载体Charon 4A，从该细胞系分离出8.3-和3.3-KBP的前病毒。限制性内切酶图谱显示，这2个前病毒克隆分别是REV-A的前病毒和REV-T的变异体。用针对Rev-T REL序列的变异型REV-T前病毒亚克隆作为杂交探针，证明REL序列不同于Schmidt-Ruppin肉瘤病毒A株、禽髓细胞瘤病毒、禽成髓细胞增殖症病毒、禽红母细胞增殖症病毒、Abelson小鼠白血病病毒和Friend红白血病病毒的推测的转化序列。REL特异性杂交探针被用来鉴定用几种限制性内切酶消化的未感染的禽类DNA中存在的一组特定序列。相应的细胞序列不像REV-T中的REL那样排列。
Reticuloendotheliosis virus strain T (REV-T) is a highly oncogenic avian retrovirus which causes a rapid neoplastic disease of the lymphoreticular system. Upon infection, this virus gives rise to 2 spp. of unintegrated linear viral DNA which are 8.3 and 5.5 kilobase pairs (kbp) long and represent the helper virus (REV-A) and the oncogenic component (REV-T), respectively. Restriction endonuclease cleavage maps of these 2 DNA components indicate that REV-T DNA has a large portion of the genome deleted with respect to REV-A DNA and a substitution .apprx. 0.8-1.5 kbp long that is unrelated to REV-A DNA. These additional sequences comprise the putative transforming region of REV-T (rel). A chicken spleen cell line transformed by REV-T produced virus which upon infection gives rise to 3 spp. of unintegrated linear viral DNA (8.3, 5.5 and 3.3 kbp). The proviruses of the 8.3- and 3.3-kbp species from this cell line were isolated by cloning in the phage vector Charon 4A. Restriction enzyme mapping showed that the 2 proviral clones are proviruses of REV-A and a variant of REV-T, respectively. A subclone of the variant REV-T provirus specific for the rel sequences of REV-T was used as a hybridization probe to demonstrate that the rel sequences are different from the putative transforming sequences of Schmidt-Ruppin Rous sarcoma virus strain A, avian myelocytomatosis virus, avian myeloblastosis virus, avian erythroblastosis virus, Abelson murine leukemia virus and Friend erythroleukemia virus. The rel-specific hybridization probe was used to identify a specific set of sequences which are present in uninfected avian DNA digested with several restriction enzymes. The corresponding cell sequences are not arranged like rel in REV-T.