Manipulation of individual viruses: Friction and mechanical properties
Manipulation of individual viruses: Friction and mechanical properties
复制标题
DOI：
10.1016/s0006-3495(97)78786-1
复制
发表时间：
1997-03-01
影响因子：
3.4
通讯作者：
Taylor, RM
中科院分区：
文献类型：
--
作者：
Falvo, MR;Washburn, S;Taylor, RM
我们提出了我们的研究结果上操纵的个别病毒使用先进的接口原子力显微镜（AFM），我们表明，病毒可以解剖，旋转，并与伟大的设施平移。我们解释了烟草花叶病毒的行为与力学模型，使明确的样品基板的横向摩擦和操纵对象的弯曲刚度之间的竞争。烟草花叶病毒在石墨上的操纵行为与在类似界面上观察到的侧向摩擦值和大分子组装体的预期抗弯刚度一致。操作单个样品的能力通过提供用于将样品定位在特定结合位点或预定义测量装置的手段来拓宽可能的研究范围。力学模型提供了一个框架，用于解释定量测量的病毒结合和机械性能，并了解成功的，无损的AFM操作微妙的样品的限制。
We present our results on the manipulation of individual viruses using an advanced interface for atomic force microscopes (AFMs), We show that the viruses can be dissected, rotated, and translated with great facility. We interpret the behavior of tobacco mosaic virus with a mechanical model that makes explicit the competition between sample-substrate lateral friction and the flexural rigidity of the manipulated object. The manipulation behavior of tobacco mosaic virus on graphite is shown to be consistent with values of lateral friction observed on similar interfaces and the flexural rigidity expected for macromolecular assemblies. The ability to manipulate individual samples broadens the scope of possible studies by providing a means for positioning samples at specific binding sites or predefined measuring devices. The mechanical model provides a framework for interpreting quantitative measurements of virus binding and mechanical properties and for understanding the constraints on the successful, nondestructive AFM manipulation of delicate samples.