ISOLATION OF CDNAS PARTIALLY ENCODING 4 XENOPUS WNT-1/INT-1-RELATED PROTEINS AND CHARACTERIZATION OF THEIR TRANSIENT EXPRESSION DURING EMBRYONIC-DEVELOPMENT
ISOLATION OF CDNAS PARTIALLY ENCODING 4 XENOPUS WNT-1/INT-1-RELATED PROTEINS AND CHARACTERIZATION OF THEIR TRANSIENT EXPRESSION DURING EMBRYONIC-DEVELOPMENT
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DOI：
10.1016/0012-1606(91)90073-c
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发表时间：
1991-02-01
影响因子：
2.7
通讯作者：
MOON, RT
中科院分区：
文献类型：
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作者：
CHRISTIAN, JL;GAVIN, BJ;MOON, RT
为了开始研究Wnt-1int-1基因家族在脊椎动物发育中的功能，我们分离了四个非洲爪哇cDNA，编码与Wnt-1int-1同源的蛋白质的部分序列。Xwnt-3、Xwnt-4、Xwnt-5A和Xwnt-8与X.laevis Wnt-1 int-1有35%~50%的氨基酸同源性，并且大多数半胱氨酸残基是保守的。XWNT-4和XWNT-3转录本只在神经细胞发育到蝌蚪阶段才能检测到。Xwnt-8的表达在原肠发育过程中可观察到，在神经形成过程中下降，在蝌蚪发育阶段检测不到。XWNT-5A转录本在卵母细胞和蝌蚪的RNA中最普遍，尽管在所研究的所有阶段都检测到低水平的表达。这些转录本表达的时间变化意味着每个Xwnt在胚胎发育过程中都扮演着独特的角色。
To begin to study the functions of the Wnt-1 int-1 gene family during vertebrate development, we have isolated four Xenopus laevis cDNAs encoding the partial sequence of proteins homologous to Wnt-1 int-1. Xwnt-3, Xwnt-4, Xwnt-5A, and Xwnt-8 demonstrate between 35 and 50% amino acid identity with X. laevis Wnt-1 int-1 and most cysteine residues are conserved. Xwnt-4 and Xwnt-3 transcripts are detected only during the neurula through tadpole stages of development. Expression of Xwnt-8 is observable during gastrulation, declines during neurulation, and is undetectable by the tadpole stage of development. Xwnt-5A transcripts are most prevalent in RNA from oocytes and tadpoles, although low level expression is detected at all stages examined. The temporal changes in expression of these transcripts imply a unique role for each Xwnt during embryogenesis.