Tropomyosin expression and dynamics in developing avian embryonic muscles
Tropomyosin expression and dynamics in developing avian embryonic muscles
复制标题
DOI：
10.1002/cm.20267
复制
发表时间：
2008-05-01
影响因子：
--
通讯作者：
Sanger, Joseph W.
中科院分区：
文献类型：
--
作者：
Wang, Jushuo;Thurston, Harold;Sanger, Joseph W.
The expression of striated muscle proteins occurs early in the developing embryo in the somites and forming heart. A major component of the assembling rnyofibrils is the actin-binding protein tropomyosin. In vertebrates, there are four genes for tropomyosin (TM), each of which can be alternatively spliced. TPM1 can generate at least 10 different isoforms including the striated muscle-specific TPM1 alpha and TPM1 kappa. We have undertaken a detailed study of the expression of various TM isoforms in 2-day-old (stage HH 10-12; 33 h) heart and somites, the progenitor of future skeletal muscles. Both TPM1 alpha and TPM1 kappa are expressed transiently in embryonic heart while TPM1 alpha is expressed in somites. Both RT-PCR and in situ hybridization data suggest that TPM1 kappa is expressed in embryonic heart whereas TPM1 alpha is expressed in embryonic heart, and also in the branchial arch region of somites, and in the somites. Photobleaching studies of Yellow Fluorescent Protein-TPM1 alpha and -TPM1 kappa expressed in cultured avian cardiornyocytes revealed that the dynamics of the two probes was the same in both premyofibrils and in mature myofibrils. This was in sharp contrast to skeletal muscle cells in which the fluorescent proteins were more dynamic in premyofibrils. We speculate that the differences in the two muscles is due to the appearance of nebulin in the skeletal myocytes premyofibrils transform into mature myofibrils. Cell Motil.
