Transformation of humic substances by the freshwater Ascomycete Cladosporium sp.
Transformation of humic substances by the freshwater Ascomycete Cladosporium sp.
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DOI：
10.1002/lno.10545
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发表时间：
2017-09
影响因子：
4.5
通讯作者：
Keilor Rojas‐Jimenez;Jérémy A. Fonvielle;Hua Ma;H. Grossart
中科院分区：
文献类型：
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作者：
Keilor Rojas‐Jimenez;Jérémy A. Fonvielle;Hua Ma;H. Grossart
真菌在淡水生态系统中的生态相关性正变得越来越明显，特别是在处理大量的聚合物有机碳时，如纤维素、甲壳素和腐殖质(HS)。我们从德国勃兰登堡的斯泰克林湖分离出了几株真菌菌株，并分析了它们降解类聚合物底物的能力。采用高效液相色谱-有机碳检测法，对淡水真菌枝孢霉转化HS的副产物进行了检测。KR14.我们证明了这种真菌同时降解和合成HS的能力，并且当介质中存在铁作为Fenton反应发生的指示剂时，转化过程被加强。此外，我们还表明，产生的HS的结构复杂性随着介质中其他聚合物的可获得性而变化。我们的研究突出了淡水子囊菌对复杂有机化合物转化的贡献。因此，这对于理解真菌对水生食物网的生态贡献以及相关的生物地球化学循环具有重要意义。
The ecological relevance of fungi in freshwater ecosystems is becoming increasingly evident, particularly in processing the extensive amounts of polymeric organic carbon such as cellulose, chitin, and humic substances (HS). We isolated several fungal strains from oligo‐mesotrophic Lake Stechlin, Brandenburg, Germany, and analyzed their ability to degrade polymeric‐like substrates. Using liquid chromatography‐organic carbon detection, we determined the byproducts of HS transformation by the freshwater fungus Cladosporium sp. KR14. We demonstrate the ability of this fungus to degrade and simultaneously synthesize HS, and that transformation processes were intensified when iron, as indicator of the occurrence of Fenton reactions, was present in the medium. Furthermore, we showed that structural complexity of the HS produced changed with the availability of other polymeric substances in the medium. Our study highlights the contribution of freshwater Ascomycetes to the transformation of complex organic compounds. As such, it has important implications for understanding the ecological contribution of fungi to aquatic food webs and related biogeochemical cycles.