LEYDIG-CELL NUMBERS, DAILY SPERM PRODUCTION, AND SERUM GONADOTROPIN-LEVELS IN AGING MEN
LEYDIG-CELL NUMBERS, DAILY SPERM PRODUCTION, AND SERUM GONADOTROPIN-LEVELS IN AGING MEN
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DOI：
10.1210/jcem-59-4-756
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发表时间：
1984-01-01
影响因子：
5.8
通讯作者：
PETTY, CS
中科院分区：
文献类型：
--
作者：
NEAVES, WB;JOHNSON, L;PETTY, CS
研究了15名年龄在20岁至48岁之间的男性和15名年龄在50岁至76岁之间的男性间质细胞数量、每日精子产量和循环激素水平的年龄相关变化。睾丸和血液样本在死于创伤或心脏病发作后15小时的尸检中获得。采用定量组织计量学方法，测定受试者双侧睾丸中戊二醛灌流的、去包膜的睾丸实质所占睾丸细胞核平均体积的比例，以确定睾丸间质细胞的数量。用相差细胞仪法测定每个个体两个固定睾丸匀浆中圆形精子细胞核的日精子产量。用放射免疫法测定心脏或大静脉血中黄体生成素、卵泡刺激素、催乳素和睾酮的含量。没有发现任何睾丸或荷尔蒙数值与死后时间之间的关系。老年男性间质细胞平均总数减少44%，与年龄呈负相关(P=-0.62)。老年男性平均血清黄体生成素水平升高2倍，并与所有男性的年龄呈正相关(P=+0.53)。血清睾酮和血清催乳素均不随年龄增长而发生明显变化。老年男性的每日精子产生量降低50%，并且与所有男性的年龄呈负相关(P=-0.76)。老年男性的血清FSH是年龄的3倍，并且与年龄呈正相关(P=+0.72)。FSH水平在精子生成率最低的男性中最高，即使在剔除年龄的影响后，FSH与每日精子生成量也呈负相关。人类睾丸对衰老的反应是不同的，在男性个体水平上，年龄对大多数睾丸特征的预测价值很弱。年龄解释了间质细胞数量变化的1/3，也解释了每天精子产量变化的1/2。在寻找正常男性睾丸结构和功能差异的原因的过程中，年龄是迄今确定的最大单一因素。
Age-related changes in Leydig cell number, daily sperm production, and circulation hormone levels were studied in 15 men between 20 and 48 yr of age, and 15 men between 50 and 76 yr of age. Testes and blood samples were obtained at autopsy < 15 h after death due to trauma or heart attack. Leydig cell number was determined by quantitative histometric estimation of the proportion of glutaraldehyde-perfused, decapsulated testicular parenchyma occupied by Leydig cell nuclei of measured average volume in both testes of each subject. Daily sperm production was determined by phase contrast cytometry of round spermatid nuclei in homogenates of both fixed testes from each individual. LH [luteinizing hormone], FSH, PRL [prolactin] and testosterone in serum from the heart or large veins were quantified by RIA [radioimmunoassay]. No relationship was detected between any of the testicular or hormonal values and postmortem time. The average total number of Leydig cells was reduced by 44% in the older men and was negatively correlated with age (P = -0.62) in all men. Mean serum LH was elevated > 2-fold in the older men and was positively correlated with age (P = +0.53) in all men. Neither serum testosterone nor serum PRL changed significantly with age. Daily sperm production was > 50% lower in older men and was negatively correlated with age (P = -0.76) in all men. Serum FSH was > 3-fold higher in the older men and was positively correlated with age (P = +0.72) in all men. The highest FSH levels were found in men with the lowest rates of sperm production, and FSH and daily sperm production were inversely correlated even after the effects of age were removed. The response of the human testis to aging is variable and the predictive value of age for most testicular characteristics is weak at the level of individual men. Age accounts for > 1/3 of the variation in Leydig cell number, and it explains for > 1/2 the variation in daily sperm production. Age is the largest single contributing factor yet identified in the search for explanations underlying the variation in testicular structure and function found in groups of normal men.