Unspecific labeling of pancreatic islets by antisera against fibroblast growth factors and their receptors
Unspecific labeling of pancreatic islets by antisera against fibroblast growth factors and their receptors
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DOI：
10.1177/002215540305100314
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发表时间：
2003-03-01
影响因子：
3.2
通讯作者：
Serup, P
中科院分区：
文献类型：
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作者：
Dichmann, DS;Rescan, C;Serup, P
用免疫组织化学方法(IHC)检测了6种不同的成纤维细胞生长因子(FGFs)在胰岛中的表达。我们在这里表明，这些抗血清对免疫印迹是有用的，但只有两种抗血清适合于IHC。通过Western blotting，这些抗血清检测到重组的FGFs。用免疫多肽预吸收可以消除检测，但不能用无关多肽进行预吸收。经免疫组化检测，发现抗FGF1和抗FGF2抗血清可特异性地标记胰岛。免疫多肽的预吸收可以取消标记。相反，抗FGF4、-FGF5、-FGF7和-FGF10抗血清对胰岛的显著染色是非特异性的，因为这种染色不能与免疫多肽的预吸收竞争。
Six distinct fibroblast growth factors (FGFs) have been detected in pancreatic islets by immunohistochemistry (IHC) using commercially available antisera. We show here that these antisera are useful for Western blotting but that only two are suited for IHC. By Western blotting, these antisera detect recombinant FGFs. Detection can be eliminated by preabsorption with immunizing peptide but not with irrelevant peptide. By IHC we find specific labeling of islets with anti-FGF1 and anti-FGF2 antisera. Labeling can be abolished by preabsorption with the immunizing peptides. In contrast, prominent staining of islets by anti-FGF4, -FGF5, -FGF7, and -FGF10 antisera is unspecific because the staining cannot be competed by preabsorption with the immunizing peptides.