MUTATIONAL ACTIVATION OF RAS GENES IS ABSENT IN PEDIATRIC OSTEOSARCOMA
MUTATIONAL ACTIVATION OF RAS GENES IS ABSENT IN PEDIATRIC OSTEOSARCOMA
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DOI：
10.1016/0165-4608(94)00115-r
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发表时间：
1995-01-01
影响因子：
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通讯作者：
SIERRASESUMAGA, L
中科院分区：
文献类型：
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作者：
ANTILLONKLUSSMANN, F;GARCIADELGADO, M;SIERRASESUMAGA, L
ras癌基因的激活在人类癌症中被发现;总体而言，在所有肿瘤的15%中观察到。本研究的目的是评估ras癌基因在骨肉瘤中的参与程度。我们对49例诊断为骨肉瘤并在我们机构接受治疗的儿童患者的肿瘤样本进行了评价。通过聚合酶链反应（PCR）、狭缝印迹法和放射性标记的特异性DNA探针，对诊断性活检的石蜡包埋肿瘤样本、新辅助化疗后的肿瘤整块切除组织以及转移灶样本进行检查，以寻找H、K和N-ras基因密码子12和61处的点突变。共研究了92个档案样本。未发现激活这些基因的点突变，提示H、K和N-ras基因第12和61密码子点突变激活在人骨肉瘤的发病机制中不起作用。由于未检测到密码子12和61的点突变，因此无法确定ras基因与临床或组织学结果之间的任何相关性。
Activation of ras oncogenes is found in human cancers; overall it is observed in 15% of all neoplasms. The purpose of this study was to assess the extent of involvement of ras oncogenes in osteosarcoma. Tumor samples from a series of 49 pediatric patients diagnosed with osteosarcoma and treated at our institution were evaluated. Paraffin-embedded tumor samples from diagnostic biopsies, from tumor en bloc resection tissue after neoadjuvant chemotherapy, and samples from metastases were examined in search of point mutations in H, K, and N-ras genes at codons 12 and 61 by means of polymerase chain reaction (PCR), slot-blotting, and radioactive labeled specific DNA probes. A total of 92 archival samples were studied. No point mutations activating these genes were found. These findings suggest that the activation by point mutations at codons 12 and 61 of the H, K, and N-ras genes does nor play a role in the pathogenesis of human osteosarcoma. Since no point mutations in codons 12 and 61 were detected, if was not possible to establish any correlation between the ras genes and clinical or histologic findings.