Zygotic loss of ZEN-4/MKLP1 results in disruption of epidermal morphogenesis in the C-elegans embryo
Zygotic loss of ZEN-4/MKLP1 results in disruption of epidermal morphogenesis in the C-elegans embryo
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DOI：
10.1002/dvdy.21455
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发表时间：
2008-03-01
影响因子：
2.5
通讯作者：
Raich, William B.
中科院分区：
文献类型：
--
作者：
Hardin, Jeff;King, Ryan;Raich, William B.
在秀丽隐杆线虫中，ZEN - 4/AEKLP1在母源上对于胞质分裂是必需的，但它最初是在合子致死、包被异常（Zen）突变体的筛选中被鉴定出来的。我们报道zen - 4(w35)纯合子在多个谱系中表现出胞质分裂的随机失败。值得注意的是，多核表皮细胞显示出定向迁移，即使细胞数量只有正常数量的一半。温度转换实验和对zen - 4::gfp表达的分析证实，zen - 4功能的表皮需求在形态发生之前。通过上皮特异性转基因驱动野生型zen - 4的表达能够挽救zen - 4突变体中的许多表皮形态发生缺陷。在表皮前体细胞中早期表达unc - 119使得这个启动子在这种情况下不适合作为神经元特异性驱动因子。我们的结果表明，合子的zen - 4功能对于表皮前体细胞的正确分裂是必需的，因此间接地对于正常形态发生是必需的，并且即使在缺乏zen - 4功能的情况下，表皮形态发生程序也惊人地稳健。
ZEN-4/AEKLP1 is required maternally for cytokinesis in Caenorhabditis elegans, but was originally identified in a screen for zygotic lethal, enclosure abnormal (Zen) mutants. We report that zen-4(w35) homozygotes exhibit stochastic failures in cytokinesis in multiple lineages. Remarkably, multinucleate epidermal cells show directional migration, even when there are as few as half the normal number of cells. Temperature shift experiments and analysis of zen-4::gfp expression confirm that the epidermal requirement for zen-4 function precedes morphogenesis. Driving expression of wild-type zen-4 by means of an epithelial-specific transgene can rescue many epidermal morphogenetic defects in zen-4 mutants. Early expression of unc-119 in epidermal precursors made this promoter unsuitable as a neuronal-specific driver in this context. Our results indicate that zygotic zen-4 function is required for correct division of epidermal precursors and, hence, indirectly for normal morphogenesis and that the epidermal morphogenetic program is surprisingly robust even in the absence of zen-4 function.