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DOI：
10.1101/2020.08.31.276642
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发表时间：
2020-09
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Tailocins是噬菌体衍生的细菌素，由于其高杀灭效率和精确的菌株特异性靶向能力，显示出作为农业抗菌剂的巨大潜力。本课组近期对植物病原菌丁香假单胞菌产生的tailocins进行了分类和表征，并对tailocins的敏感性进行了研究。在此，我们将对这些实验进行扩展，以检验预防性使用tailocin是否可以预防丁香假单胞菌对benthamiana的感染。两B728a。具体地说，我们证明了多个菌株可以产生tailocins来预防菌株B728a的感染，并设计了一个缺失突变体来证明tailocins靶向是这种保护作用的原因。最后，我们提供的证据表明，遗传抗性突变不能解释少数情况下，tailocins不能预防感染。我们的研究结果扩展了以前关于预防性使用tailocins抗植物病原体的报道，并建立了一个模型系统来测试和优化tailocins用于预防植物病原体感染的预防治疗。
Tailocins are phage-derived bacteriocins that demonstrate great potential as agricultural antimicrobials given their high killing efficiency and their precise strain-specific targeting ability. Our group has recently categorized and characterized tailocins produced by and tailocin sensitivities of the phytopathogen Pseudomonas syringae, and here we extend these experiments to test whether prophylactic tailocin application can prevent infection of Nicotiana benthamiana by P. syringae pv. syringae B728a. Specifically, we demonstrate that multiple strains can produce tailocins that prevent infection by strain B728a and engineer a deletion mutant to prove that tailocin targeting is responsible for this protective effect. Lastly, we provide evidence that heritable resistance mutations do not explain the minority of cases where tailocins fail to prevent infection. Our results extend previous reports of prophylactic use of tailocins against phytopathogens, and establish a model system with which to test and optimize tailocin application for prophylactic treatment to prevent phytopathogen infection.