Identification of genes in Rhizobium leguminosarum bv. trifolii whose products are homologues to a family of ATP-binding proteins.
Identification of genes in Rhizobium leguminosarum bv. trifolii whose products are homologues to a family of ATP-binding proteins.
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DOI：
10.1099/00221287-143-4-1389
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发表时间：
1997-04
期刊：
影响因子：
1.5
通讯作者：
Jaroslaw E. Król;A. Skorupska
中科院分区：
文献类型：
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作者：
Jaroslaw E. Król;A. Skorupska
根瘤菌与其宿主之间的特异性相互作用需要许多影响共生早期和晚期步骤的基因。三个新的基因，命名为prsD，prsE（蛋白质分泌）和orf 3，被确定为exo 133突变附近的粘粒携带的基因组DNA的豆科根瘤菌bv。三叶草TA 1. prsDE基因与编码来自欧文氏菌的ABC转运蛋白PrtDE和来自铜绿假单胞菌的AprDE的基因具有显著的同源性，所述ABC转运蛋白PrtDE和AprDE在这些细菌中输出蛋白酶。PrsD显示至少五个潜在的跨膜疏水区和一个含有ATP/GTP结合盒的大亲水结构域。PrsE在N-末端部分只有一个潜在的跨膜疏水结构域，被认为在转运系统中起辅助因子的作用。ORF 3与PrtF和AprF一样，具有典型的N-末端信号序列，但与这些蛋白质没有同源性。将卡那霉素抗性盒插入到R.豆科植物三叶草TA 1野生型菌株产生了产生正常量的外泌多糖但在三叶草植物的发酵中无效的突变体。
The specific interaction between rhizobia and their hosts requires many genes that influence both early and late steps in symbiosis. Three new genes, designated prsD, prsE (protein secretion) and orf3, were identified adjacent to the exo133 mutation in a cosmid carrying the genomic DNA of Rhizobium leguminosarum bv. trifolii TA1. The prsDE genes share significant homology to the genes encoding ABC transporter proteins PrtDE from Erwinia chrysanthemi and AprDE from Pseudomonas aeruginosa which export the proteases in these bacteria. PrsD shows at least five potential transmembrane hydrophobic regions and a large hydrophilic domain containing an ATP/GTP binding cassette. PrsE has only one potential transmembrane hydrophobic domain in the N-terminal part and is proposed to function as an accessory factor in the transport system. ORF3, like PrtF and AprF, has a typical N-terminal signal sequence but has no homology to these proteins. The insertion of a kanamycin resistance cassette into the prsD gene of the R. leguminosarum bv. trifolii TA1 wild-type strain created a mutant which produced a normal amount of exopolysaccharide but was not effective in the nodulation of clover plants.