Tissue engineered microsphere-based matrices for bone repair: design and evaluation
Tissue engineered microsphere-based matrices for bone repair: design and evaluation
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DOI：
10.1016/s0142-9612(01)00137-5
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发表时间：
2002-01-01
期刊：
影响因子：
14
通讯作者：
Laurencin, CT
中科院分区：
文献类型：
--
作者：
Borden, M;Attawia, M;Laurencin, CT
由于当前移植材料的局限性，需要合成替代品来替代传统骨移植物。我们的方法是使用微球技术设计聚合物基接枝替代品。采用聚丙交酯-乙交酯共聚物微球的无规填充设计了凝胶微球基质和烧结微球基质，以形成三维多孔结构，并对这些方法进行了评价，分析了基质组成和加工过程的影响。通过扫描电子显微镜和孔隙率测定法对基质进行结构评估，并通过压缩测试进行生物力学评估。评价揭示了凝胶微球基质的高模量和烧结微球基质的多功能性。凝胶微球基质中含有羟基磷灰石颗粒，杨氏模量为1651 MPa，但通过SEM进行的结构分析显示，孔隙系统对骨向内生长不太理想。通过将聚合物微球热熔合成三维阵列来制造不含羟基磷灰石颗粒的烧结微球基质，其具有互连性和241（+/-82）349（+/-89）MPa的模量范围。烧结的微球基质表现出连接的孔隙系统和松质骨中等范围的机械性能。孔隙率数据表明，矩阵孔径直接与微球直径变化，而孔体积是独立的微球直径的范围内的直径检查。基于微球的基质显示出作为骨修复的聚合物替代物的前景。(C)2001爱思唯尔科技有限公司版权所有。
The need for synthetic alternatives to conventional bone grafts is due to the limitations of current grafting materials. Our approach has been to design polymer-based graft substitutes using microsphere technology. The gel microsphere matrix and the sintered microsphere matrix were designed using the random packing of poly(lactide-co-glycolide) microspheres to create a three-dimensional porous structure,The evaluation of these methods dealt with analysis of effects of matrix composition and processing. Matrices were evaluated structurally by scanning electron microscopy and porosimetry, and biomechanically by compression testing. The evaluation revealed the high modulus Of the gel microsphere matrix and the versatility of the sintered microsphere matrix. The gel microsphere matrix incorporated hydroxyapatite particles and had a Young's modulus of 1651 MPa, but structural analysis through SEM revealed a pore system less optimal for bone in-growth. The sintered microsphere matrices were fabricated without hydroxyapatite particles by thermally fusing polymeric microspheres into a three-dimensional array, possessing interconnectivity and a modulus range of 241 (+/- 82) 349 (+/- 89) MPa. The sintered microsphere matrix demonstrated a connected pore system and mechanical properties in the mid-range of cancellous bone. Porosimetry data indicated that matrix pore diameter varied directly with microsphere diameter, while pore volume was independent of microsphere diameter in the range of diameters examined. The microsphere-based matrices show promise as polymeric substitutes for bone repair. (C) 2001 Elsevier Science Ltd. All rights reserved.