Sterigmatocystin induced apoptosis in human pulmonary cells in vitro
Sterigmatocystin induced apoptosis in human pulmonary cells in vitro
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杂色曲霉素体外诱导人肺细胞凋亡
DOI：
10.1016/j.etp.2017.07.002
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发表时间：
2017-10-02
影响因子：
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通讯作者：
Zhang, Xianghong
中科院分区：
文献类型：
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作者：
Cui, Jinfeng;Wang, Juan;Zhang, Xianghong
Sterigmatocystin （ST）是公认的潜在致癌物、诱变物和致畸物。研究表明，ST可在体内诱导小鼠肺腺癌，并在体外诱导人支气管上皮细胞系（sea -2 B细胞）和人肺癌细胞系（A549细胞）的DNA损伤和细胞周期阻滞。此外，ST还能诱导其他几种细胞的G(2)阻滞（G2期细胞周期阻滞）。细胞周期阻滞可能是ST常见的毒性作用之一，细胞可能因细胞周期阻滞而发生凋亡或死亡，我们想知道凋亡是否是ST在体外不同细胞中的另一种常见作用。本研究采用3-（4,5-二甲基噻唑-2-基）- 2,5 -二苯基溴化四唑（MTT）和流式细胞术（FCM）研究ST对A549细胞和beas - 2b细胞增殖和凋亡的影响。MTT结果显示，经ST处理24 h后，A549和BEAS-2 B细胞体外增殖均受到抑制。经ST处理后，FCM细胞凋亡增加。western blot检测ST处理后Bcl-2的下调、Bax的上调和caspase-3的激活。本研究结果与我们之前的研究结果一致，表明ST在体外诱导不同细胞的凋亡可能是一种共同的作用。
Sterigmatocystin (ST) is generally recognized as a potential carcinogen, mutagen and teratogen. Studies showed that ST could induce adenocarcinoma of lung in mice in vivo and DNA damage, cell cycle arrest in a human immortalized bronchial epithelial cell line (SEAS-2 B cells) and a human lung cancer cell line (A549 cells) in vitro. Besides, ST could induce G(2) arrest (cell cycle arrest in G2 phase) in several other cells. Cell cycle arrest may be one of the common toxic effects of ST. As cells may undergo apoptosis or death due to cell cycle arrest, we wondered whether apoptosis is another common effect of ST in different cells in vitro. In the present study, we studied the effects of ST on proliferation and apoptosis in A549 cells and BEAS-2 B cells with 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and flow cytometric analysis (FCM). The MTT results showed that proliferation inhibition following ST treatment for 24 h was observed in both A549 and BEAS-2 B cells in vitro. And increased apoptosis by FCM was also found after ST treatment. Down-regulation of Bcl-2, up-regulation of Bax and the activation of caspase-3 after ST treatment were detected by western blotting analyses. The results in the present study are consistent with our previous results, which indicated that inducing apoptosis may be a common effect of ST in different cells in vitro.