Comparison of ectopic bone formation process induced by four calcium phosphate ceramics in mice
Comparison of ectopic bone formation process induced by four calcium phosphate ceramics in mice
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四种磷酸钙陶瓷诱导小鼠异位骨形成过程的比较
DOI：
10.1016/j.msec.2016.06.097
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发表时间：
2017-01-01
影响因子：
7.9
通讯作者：
Zhang, Xingdong
中科院分区：
文献类型：
--
作者：
Tang, Zhurong;Tan, Yanfei;Zhang, Xingdong
相组成对磷酸钙陶瓷的生物降解起着关键作用，而生物降解又影响着磷酸钙陶瓷的骨诱导能力。体内生物学机制仍然知之甚少。在这项研究中，四种类型的多孔CaP陶瓷进行了研究，即，羟基磷灰石（HA），β-磷酸三钙（TCP），和双相磷酸钙BCP 1和BCP 2，HA β-TCP的比例分别为70/30和30/70。将四种陶瓷植入小鼠大腿肌肉内16周。在基因、蛋白质和组织水平上评估材料诱导的纵向异位骨形成过程。组织学分析显示，BCP 2是16周后促进新骨形成的唯一一组。在生物降解的显微CT分析中，BCP 2组由于新骨形成而具有最小的孔隙度增加，导致材料密度显著升高。而不是一个稳定的增加，多个峰值中观察到的时间基因表达模式。基因表达结果通过相应蛋白的免疫组化染色进一步证实。在靶基因中，Osterix和I型胶原依次被激活。与非骨形成组相比，骨诱导BCP 2组显示出更早且显著更高的BMP 2、BMPRIA和OPG表达峰值。这些发现揭示了这些成骨基因表达峰的出现时间和幅度在骨诱导过程中可能是至关重要的。(C)2016爱思唯尔B. V.保留所有权利。
Phase composition played a key role in the biodegradation of calcium phosphate ceramics (CaP), which in turn influences the osteoinductive ability. The in vivo biological mechanism is still poorly understood. In this study, four types of porous CaP ceramics were investigated, namely, hydroxyapatite (HA), beta-tricalcium phosphate (TCP), and biphasic calcium phosphates BCP1 and BCP2, with HA to beta-TCP ratios of 70/30 and 30/70, respectively. The four types of ceramics were implanted into thigh muscle of mice for 16 weeks. Longitudinal ectopic bone formation process at gene, protein, and tissue level induced by the material was assessed. Histological analysis revealed that BCP2 was the only group that had promoted new bone formation after 16 weeks. In micro-CT analysis of biodegradation, the BCP2 group had the least increment of porosity due to the new bone formation, resulting in a significant elevation in material density. Instead of a steady increase, multiple peaks were observed in most of the temporal gene expression patterns. The gene expression results were further confirmed by immunohistochemical staining of the corresponding proteins. Among the target genes, Osterix and type I collagen were activated successively. The osteoinductive BCP2 group showed earlier and significantly higher peaks in BMP2, BMPRIA, and OPG expressions than non-bone forming groups. These findings revealed that the occurrence time and magnitude of these osteogenetic gene expression peaks can be crucial in the osteoinduction process. (C) 2016 Elsevier B.V. All rights reserved.