Pericardial patch venoplasty heals via attraction of venous progenitor cells.
Pericardial patch venoplasty heals via attraction of venous progenitor cells.
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DOI：
10.14814/phy2.12841
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发表时间：
2016-06
影响因子：
2.5
通讯作者：
Dardik A
中科院分区：
文献类型：
--
作者：
Bai H;Wang M;Foster TR;Hu H;He H;Hashimoto T;Hanisch JJ;Santana JM;Xing Y;Dardik A
心包贴片在心血管手术中常用来闭合血管。在动脉中，斑块聚集了动脉祖细胞；我们假设在没有动脉压力的情况下，静脉斑块会积聚静脉祖细胞。我们建立了一种新型的大鼠下腔静脉（IVC）切开模型，并用心包补片修复。第7天，细胞浸润斑块形成厚的新生内膜；一些细胞是CD34+/VEGFR2+和CD31+/Eph‐B4+，这与愈合贴片中静脉身份的发展一致。与动脉斑块相比，静脉斑块在第7天未出现假性动脉瘤时内膜厚度增加。添加动静脉瘘（AVF）以增加贴片上的血流量，导致贴片内膜厚度和增殖减少，但动静脉瘘结扎后内膜厚度不可逆转。这些结果表明，大鼠静脉补片成形术是一种新的侵袭性静脉内膜增生模型。
Pericardial patches are commonly used during cardiovascular surgery to close blood vessels. In arteries, patches accumulate arterial progenitor cells; we hypothesized that venous patches would accumulate venous progenitor cells, in the absence of arterial pressure. We developed a novel rat inferior vena cava (IVC) venotomy model and repaired it with a pericardial patch. Cells infiltrated the patch to form a thick neointima by day 7; some cells were CD34+/VEGFR2+ and CD31+/Eph‐B4+ consistent with development of venous identity in the healing patch. Compared to arterial patches, the venous patches had increased neointimal thickness at day 7 without any pseudoaneurysms. Addition of an arteriovenous fistula (AVF) to increase blood flow on the patch resulted in reduced patch neointimal thickness and proliferation, but neointimal thickness was not reversible with AVF ligation. These results show that rat patch venoplasty is a novel model of aggressive venous neointimal hyperplasia.