Chromosomal fusions, but not chromosomal inversions, activate a PCH-2 dependent checkpoint that promotes crossover formation in C. elegans.
Chromosomal fusions, but not chromosomal inversions, activate a PCH-2 dependent checkpoint that promotes crossover formation in C. elegans.
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DOI：
10.17912/micropub.biology.000839
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发表时间：
2023
影响因子：
--
通讯作者：
Bhalla, Needhi
中科院分区：
文献类型：
--
作者：
Patel, Bhumil;Grobler, Maryke;Bhalla, Needhi
减数分裂交叉在配子发生过程中促进精确的染色体分离。在线虫中，高度保守的AAA ATPase PCH-2确保同源染色体至少有一个交叉，防止减数分裂缺陷。PCH-2定位于减数分裂染色体，当减数分裂重组中存在缺陷时，这种定位会延长，这表明PCH-2在对缺陷的反应中发挥了作用。在这里，我们表明，与其他系统不同的是，PCH-2在有染色体倒位的减数分裂染色体上不存在，但在有完整的染色体融合时确实存在。此外，这种持久性与交换的增加相关，表明PCH-2‘S定位在染色体上促进了交换的形成。
Meiotic crossovers promote accurate chromosome segregation during gametogenesis. In C. elegans , a highly conserved AAA ATPase, PCH-2, ensures that homologous chromosomes have at least one crossover, preventing meiotic defects. PCH-2 localizes to meiotic chromosomes and this localization is extended when there are defects in meiotic recombination, suggesting a role in responding to defects. Here, we show that, unlike in other systems, PCH-2 does not persist on meiotic chromosomes when there are chromosomal inversions but does persist when there are whole chromosome fusions. Moreover, this persistence correlates with an increase in crossovers, demonstrating that PCH-2’s localization to chromosomes promotes crossover formation.