Evaluation of an ovine testis cell line (OA3.Ts) for propagation of capripoxvirus isolates and development of an immunostaining technique for viral plaque visualization
Evaluation of an ovine testis cell line (OA3.Ts) for propagation of capripoxvirus isolates and development of an immunostaining technique for viral plaque visualization
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DOI：
10.1177/104063870701900505
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发表时间：
2007-09-01
影响因子：
1.5
通讯作者：
Kitching, R. Paul
中科院分区：
文献类型：
--
作者：
Babiuk, Shawn;Parkyn, Geoff;Kitching, R. Paul
对绵羊睾丸细胞系（OA3.Ts）进行了评价，并与原代羔羊肾（LK）细胞进行了比较，以确定其在羊痘病毒增殖和滴定中的实用性。在低（< 33）和高（34-36）传代水平下的0A3.Ts细胞生长动力学和形态学的比较表明两种特征的差异。然而，在低代和高代OA3.Ts细胞中测定的病毒滴度与使用LK细胞获得的病毒滴度相当。羊痘病毒感染OA3.Ts和LK细胞导致类似的细胞病变效应，这允许在用羊痘病毒特异性抗血清免疫染色后检测离散的病毒噬斑。
An ovine testis cell line (OA3.Ts) was evaluated and compared with primary lamb kidney (LK) cells for its utility in capripoxvirus propagation and titration. A comparison of OA3.Ts cell growth kinetics and morphology at low (< 33) and high (34-36) passage levels indicated a difference in both characteristics. However, viral titers determined in low and high passage OA3.Ts cells were comparable with those obtained using LK cells. Capripoxvirus infection of OA3.Ts and LK cells resulted in a similar cytopathic effect, which allowed for the detection of discrete viral plaques following immunostaining with capripoxvirus-specific antiserum.